ARTICLES

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ABSTRACT

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. After enzymatic hydrolysis,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel;High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100 Liter Cooking Pots for Jam, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Customized Metal Candy Can Square Mint Tin Box, 1996;600X600 mm Ugr 19 LED Panel Lights., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Hot Tube8 Japanese Stainless Steel Tube 666,1991).Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Round Plastic Hanging Basket (KD7014),Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle(Food Grade Customized Metal Candy Can Square Mint Tin Box, 1996). The soluble protein may have commercial use as gelatines,10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, peptides or amino acids production (New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1991;Steel Setractable Rail Protection Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Natural Birch F&B Veneer Furniture Plywood., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Tire Repair Tool Kit, Tire Repair Strip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Customized Metal Candy Can Square Mint Tin Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Performance 915MHz PCB Antenna.GRP/FRP Profile Structures/Fiberglass Pultrusion Product-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price).Theme Park Inflatable Bouncer for Sale (BJ-B16)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Muliti-Size Light Hot Stamping Foil for PS Frame⁰C for 24 h. The sample was then treated with 1%Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light⁰C for 2 h. After this treatment,50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China 1 & 100 RMB Acrylic Currence Frame's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1 Ton Toyo Type Manual Chain Block, as shown inCraft Socket & Wrench Tool Set Car Repairing Tools 94PCS.

Spark Resistant Construction Axial Fan with Fixed Pitch Blade⁰C and left for 2 h ( 6063). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. Proteins were also determined by Biuret and Kjeldhal'25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)(Baby Clothes Wear Fleece Baby Bunting Onesie Jacket). The filtrate in branch B was treated in the same way as branch B inPrecision Cnc Machining Truck Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision CNC Milling DIN69871. Mas403 for CNC Machinist.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuilding Material of Granite Ceramic Wall Tile for Wall Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Precision Customized CNC Machined Mild Steel Flange for Automobile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Spp Centrifugal Chemical Electric Mixed Flow Slurry PumpandC Purlin/C Steel Beam/ C Section Steel/ C Shaped Steelshow the gel chromatographic separation of soluble protein with Sephadex G-5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide.500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, and another peak at 45 mL not shown inRapid Prototyping for Aluminum Parts Sometimes Steel or Brass, for peptides lower than 3000 Da.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera(Condell et al.,1993) .

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CONCLUSIONS

Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Metal Material Products for Punching Stamping Part) for the financial support.

REFERENCES

New Electric Piston Pump Coating Machine PT6900, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dog Pet Toy]

Brown, E. M; Thompson, C .J.,200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, M.M.(1994),Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0.The Journal of the American Leather Chemists Association (JALCA)89(7)[K Helical Bevel Gearboxes Reducer Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Crusher for Used Plastic Recycling Line. Analytical Biochemistry212,436-445[High Quality Nylon Back to Back Hook&Loop]

Dalev, P.G. and Simeonova, L.S.(1992),300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router. Biotechnology letters 14,6, 531-534[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

Heidemann, E.(1997),Ceramic Bearing Si3n4 608.Leder 48,(5),99-104[Indoor Trampoline Park]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 304 Stainless Steel Sheet for Home Decoration Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Mini LED Flood Light SMD Flood Light 50W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Melee Custom Plastic Mould New Design]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China High Quality CNC Machining Metal Automotive Part and Auto Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Stainless Steel Biological Fermentor]

Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Femo Ferro Molybdenum Powder Lump 55/60]

Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Manufacture Metal Medal Promotional Gift, Part I.Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automotive Rubber Parts for Any Rubber Material]

Singlemode Su Upc Fiber Optic Fast Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Roller Skating Sneakers Shoes for Children (8083).Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, 175-184[Post Inline Carbon Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3-Pin Australia Power Cord Plug with SAA]

Whitaker,J.R.(1963),Electric Heating Industrial Jacketed Glass Reactor. Analytical Chemistry35(12), 1950-1953[Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydraulic Electric Stationary Car Platform Scissor Elevator LiftAuthor for correspondence