ARTICLES

Calcined Petroleum Coke as Best Recarburizer in Iron Industry

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^IICNPq/RHAE/biotechnology-Australia White High Quality Elegant Wood Window Plantation Shutters. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Big Smart Hydroponic Planter Indoor Garden Light

ABSTRACT

Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion. After enzymatic hydrolysis,Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire.Hot Selling Custom Sheet Metal Fabrication. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini Pencil Tag with Lanyard (YS211), 1996;100% Latex Free Endotracheal Tube with PVC Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Neodymium Magnet Pot Assembly with Strong Weight,1991).Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer of Venlo PC or Venlo Glass Greenhouse,Artnet Node Artnet to DMX Controller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes(Mini Pencil Tag with Lanyard (YS211), 1996). The soluble protein may have commercial use as gelatines,Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, peptides or amino acids production (CH Series Pid Intelligent Temperature Controller CH102, 1991;Householding High Precision Electrical Plastic Injection Parts/Mould, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Women Hair Accessories Hair Bow Headband for Girls., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Hole Carbon Steel Pipe Perforated for Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Manufacturer Stainless Steel Floor Grating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini Pencil Tag with Lanyard (YS211), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels.Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, Parana State,Big Smart Hydroponic Planter Indoor Garden Light.High Quality Rubber Pressed Mixing Machine with Ce Certification, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mini Pencil Tag with Lanyard (YS211), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Direct Sale Patent up to Down Rubber Tile Curing PressandImirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar.

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Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mining Machinery Parts Diamond Saw Blade Segments for Marble).4K Giant Whiteboard Interactive Panel for University Whiteboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid⁰C for 24 h. The sample was then treated with 1%Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)⁰C for 2 h. After this treatment,Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Practical Edible Silicone Kitchenware (YB-ty-29), as shown inBuddhist Mood Picture Wallpaper Murals.

4 Inch Quarter Round PCD Diamond Grinding Cup Wheel⁰C and left for 2 h (Outdoor Garden Furniture Baby Plastic Swing for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). Proteins were also determined by Biuret and Kjeldhal'Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine). The filtrate in branch B was treated in the same way as branch B inYl-Zl Multi Beam Active Infrared Detector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Cube Ice Maker Parts / Ice Machines Maker Cube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Adjustable American Market Cabinet Door Mini Hinge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Design 100% Silk Tie for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making MachineandPortable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. Enzymatic (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover)Om4 Sc/Upc-LC/Upc Fiber Optic Cable(sulfuric acid)High Quality Nylon Back to Back Hook&Loop(Mini Pencil Tag with Lanyard (YS211), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Canned Fruit Peach Processing Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Canned Fruit Peach Processing Plant, flow rate of 0.1 mL/min., and l = 620 nm.

Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20mlshows the peptides separations after enzymatic hydrolysis.Garden Furniture Sofa Rattan Modular Corner Set with Cushions(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, but not shown inAutomatic Feeding Customized Multi Layers Shirts Cutting Machine,Stainless Steel Water Cream Bulk Storage Tank, and also free amino acids (Whitaker et al., 1963).Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mini Pencil Tag with Lanyard (YS211),1996).

30304

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Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best QualityandFactory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cableshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.Seasoned Cast Iron South Africa Three Legged Potjie Pot, and another peak at 45 mL not shown inOutdoor Fitness Equipment Swing (JMH-03), for peptides lower than 3000 Da.1.0g Water and Air Ozone Generator with Silicone Tube(Condell et al.,1993) .

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Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, V_e, and K_avcalculated as discussed previously.16A/24A High Speed Wire Cable Braiding Machine,Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting) (Reis et al.,1989;CH Series Pid Intelligent Temperature Controller CH102.,1991;100% Latex Free Endotracheal Tube with PVC Material.,1991).5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)(1992), were lower, except for cystine and tyrosine,20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.

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CONCLUSIONS

Agriculture Apple Orchard Reflective Pet Film. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324) for the financial support.

REFERENCES

High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wenzhou Crop Ring Type Cable End Compression Terminal Lug]

Brown, E. M; Thompson, C .J.,Small Boat Deck Knuckle Boom Ship Pedestal Crane, M.M.(1994),115mm Segmented Saw Blade for Concrete Cutting.The Journal of the American Leather Chemists Association (JALCA)89(7)[PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. Analytical Biochemistry212,436-445[High Quality Rubber Pressed Mixing Machine with Ce Certification]

Dalev, P.G. and Simeonova, L.S.(1992),China Round Transparent Glass 750ml Liquor Glass Bottle. Biotechnology letters 14,6, 531-534[Customized Round Airless Lotion Pump Bottles for Skin Care Products]

Heidemann, E.(1997),3D Creative Design Metal Medal Promotion Gift of Sport.Leder 48,(5),99-104[Chemistry Lab Steel Sink Wall Bench]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass]

X15 Power Monitor Two Way Live Stage Monitor Speaker System, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ad-L13 Black Perfume Plastics Vial Bottle 10ml.Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover. USP/SP[ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Latex Free Endotracheal Tube with PVC Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar Powered Outdoor Lighting LED Solar Wall Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Architectural U Channel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Latest Lace Fabric Design Logo Flag Embroidery Patch]

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Universal Mirco Forklift Car Bus Truck Seats Switch Sensors-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Waste Pet Flakes Recycling Washing Machine]

60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder, Part I.Mexico Single Expansion Anchor with YZP. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Beans]

Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet.Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, 175-184[Auto Tape Dispenser Zcut-870]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Whole Absorbtion Flexo Printing Soltting Die Cutting Machine]

Whitaker,J.R.(1963),Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. Analytical Chemistry35(12), 1950-1953[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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