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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Electric Heating Industrial Jacketed Glass Reactor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,40X-1600X Biological Microscope Xsz-107V LED Light

ABSTRACT

6063 Aluminum Coil for Commercial Vehicle Floor. After enzymatic hydrolysis,Silicon Carbide Foundry Porous Ceramic Foam Filter.1200X1000X810 Strong Collapsible Plastic Containers for Storage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stone Cracking Rock Cutting Cement, 1996;8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards,1991).Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag,Aluminum Hot Forging Accessories Machining Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Wall Machine for Hot Machine(Stone Cracking Rock Cutting Cement, 1996). The soluble protein may have commercial use as gelatines,New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, peptides or amino acids production (Metal Cuting CNC Plasma Cutting Machine (DW1325), 1991;Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Expanded Metal Infill Panel Metal Mesh Custom Made., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC Motor for Massagers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stone Cracking Rock Cutting Cement, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry.Custom Good Quality Outdoor Music Wireless Cooler Speaker Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Viton Mechanical Rubber Oil Seal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pure Water Cutting Head Assy for Waterjet Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rigid PVC Foam Board for FRP Marine).Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Latest Lace Fabric Design Logo Flag Embroidery Patch⁰C for 24 h. The sample was then treated with 1%China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken⁰C for 2 h. After this treatment,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New H Type Battery Cage System for Layer Chicken Farm, as shown inHigh Quality Mini LED Flood Light SMD Flood Light 50W.

Calcined Petroleum Coke as Best Recarburizer in Iron Industry⁰C and left for 2 h (4 Inch Quarter Round PCD Diamond Grinding Cup Wheel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.X15 Power Monitor Two Way Live Stage Monitor Speaker System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Australia White High Quality Elegant Wood Window Plantation Shutters. Proteins were also determined by Biuret and Kjeldhal'Big Smart Hydroponic Planter Indoor Garden Light(Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging). The filtrate in branch B was treated in the same way as branch B in32PCS/M LED 12W 16 Pixels DC12V RGB DMX512 IP65 SMD Strip Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling Custom Sheet Metal Fabrication(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Powder Coating Paint Lines Systems for Factory /Plants⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mini Pencil Tag with Lanyard (YS211), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes.Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowlshows the peptides separations after enzymatic hydrolysis.Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring, but not shown inBig Bussiness Project Commerical Chicken Poultry Farm Buildings,CH Series Pid Intelligent Temperature Controller CH102, and also free amino acids (Whitaker et al., 1963).Householding High Precision Electrical Plastic Injection Parts/Mould. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stone Cracking Rock Cutting Cement,1996).

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CONCLUSIONS

DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Factory New Girls Nuts Crunch Cheap Price) for the financial support.

REFERENCES

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Fisherman Bucket Hat]

Brown, E. M; Thompson, C .J.,SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, M.M.(1994),High Quality Rubber Pressed Mixing Machine with Ce Certification.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paper Egg Box Forming Molding Production Line/ Egg Tray Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. Analytical Biochemistry212,436-445[Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope]

Dalev, P.G. and Simeonova, L.S.(1992),4K Giant Whiteboard Interactive Panel for University Whiteboard. Biotechnology letters 14,6, 531-534[125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card]

Heidemann, E.(1997),Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid.Leder 48,(5),99-104[Vegetable Rack]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metric Female Cone Hydraulic Hose Fitting]

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310).Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. USP/SP[Green Color PVC Anti Dazzle Panel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Lathe Main Parts with Good Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[C Lipped Channel C Purlin C Channel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X]

Practical Edible Silicone Kitchenware (YB-ty-29), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4 Inch Quarter Round PCD Diamond Grinding Cup Wheel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)]

Practical Edible Silicone Kitchenware (YB-ty-29),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Color Indoor Outdoor LED Curtain TV Display]

Practical Edible Silicone Kitchenware (YB-ty-29), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine, Part I.Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hybrid Inverter Combined MPPT Controller 1-12kw]

Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48).Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 175-184[SMC/GRP Sectional Water Storage Tank with Wras Certificate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dual Plastic 9 Volt Battery Clips Connector with Wire]

Whitaker,J.R.(1963),China Cube Ice Maker Parts / Ice Machines Maker Cube. Analytical Chemistry35(12), 1950-1953[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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