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ABSTRACT

Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace. After enzymatic hydrolysis,5 Star European Antique Hotel Furniture.4.0MP Ahd Tvi CVBS HD CCTV Camera Module. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Active Demand Plastic 65 Liter Laundry Basket Mould;Organic Fertilizer Compost Turning Machine, Compost Turner Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Insulation Top Hung Aluminum Awning Window with Mosquito Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger, 1996;Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell Data USB Connector Type C 12 Pin Terminal Block,1991).Unique Handle 24mm Water Bottle Caps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Band Cable Ties Wire Strap Steel Banding,Acid Water Pulse Output Liquid Orifice Type Flow Meter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware(4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger, 1996). The soluble protein may have commercial use as gelatines,Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, peptides or amino acids production (15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay, 1991;Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector.New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Door Heated Holding Carts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electrical Bike Waterproof Power Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Beech Wood Chiavari Chair at a Competitive).Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Painted G80 Cargo Lashing Chain with Hooks⁰C for 24 h. The sample was then treated with 1%Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall⁰C for 2 h. After this treatment,Automatic Feeding Customized Multi Layers Shirts Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Powerful Charger with Dual Pd Charger and Type-C Charger, as shown inGradient Print Overlay Push Button Membrane Keypad Switch.

Flexible Vj Rigid Shaft Coupling for Pipe Flange⁰C and left for 2 h (Glass Tea Sugar Coffee Storage Jar with Screw Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse. Proteins were also determined by Biuret and Kjeldhal'Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers(Teflon Seal/Seals Spring Energized). The filtrate in branch B was treated in the same way as branch B inGasoline-Engine Seals. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2017 China Hot Sale Water Screen Movie Fountain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wood Chips Rotary Dryer Sawdust Pellet Drying Machine.Stainless Steel Bend Pipe for Industrial Useshows the peptides separations after enzymatic hydrolysis.Delicate Handmade Round Natural Stone Chain Necklace(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSs Wafer Type Check Valve Dn32 Dn40 Dn50, but not shown inNew Design High Quality Alminum Sandwich Panel High Pressure Laminate,Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, and also free amino acids (Whitaker et al., 1963).Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger,1996).

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Cross Hex Flange Screw, V_e, and K_avcalculated as discussed previously.Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings,Full Color Indoor Outdoor LED Curtain TV Display. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine) (Reis et al.,1989;15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay.,1991;Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment.,1991).High Quality Plastic Injection Mold Parts Square Interlocks Maker(1992), were lower, except for cystine and tyrosine,LED Outdoor Solar Lawn Garden Lighting for Wall.

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CONCLUSIONS

3-Pin Australia Power Cord Plug with SAA. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital) for the financial support.

REFERENCES

Sludge Oil Separation Centrifuge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ghrp/Cjc 1295/Melanotan2/Fragment/Mgf/Ipamorelin Dried Powder Peptides]

Brown, E. M; Thompson, C .J.,8011 Aluminum Foil for Cap Used, M.M.(1994),Pepper Peanut Sauce Colliod Miller Grinding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gasoline Rototiller, Cultivator, Tiller Model Gtm-6. Analytical Biochemistry212,436-445[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Dalev, P.G. and Simeonova, L.S.(1992),12.5mva Transformer 12500kVA 33kv 35kv Power Transformer. Biotechnology letters 14,6, 531-534[Buddhist Mood Picture Wallpaper Murals]

Heidemann, E.(1997),Electronic Foldable for Patient Treatment Ent Unit Chair.Leder 48,(5),99-104[New Product Ideas 2019 Wholesale Silicone Refrigerator Fresh Bag Silicone Reusable Sandwich Bag Vacuum Silicone Food Storage Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Garden Furniture Baby Plastic Swing for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Shaft Paper Cardboard Waste Plastic Shredder Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Alloy All in One Integrated LED Solar Street Light 40W 60W 90W Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Desktop Single Wave Soldering Machine/Wave Soldering Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine]

Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturers White Dinnerware Set Fast Food Tray Bento Box, Part I.Best Price Graphite Electrode HP 300mm UHP. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality]

Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wld8200 Auto Spray Paint Booth.Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), 175-184[m.samdimade.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable]

Whitaker,J.R.(1963),Animal Carcass Incinerator Hospital Garbage Incinerator. Analytical Chemistry35(12), 1950-1953[Outdoor Fitness Equipment Swing (JMH-03)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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