ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk

ABSTRACT

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle. After enzymatic hydrolysis,Low Power Consumption Silicone Sex Doll Vending Machine.Toughened Glass Disc Suspension Insulator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller;63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Round Airless Lotion Pump Bottles for Skin Care Products, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Shape Dinner Football Gift Bowl with Cover Plate, 1996;Professional Exporter of Wheat Flour Mill Plant (80t)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube,1991).Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Alloy Rack for Conductor, Wire, Cable,Outdoor Accessories Fishing Hunting Masks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(Round Shape Dinner Football Gift Bowl with Cover Plate, 1996). The soluble protein may have commercial use as gelatines,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, peptides or amino acids production (Cast Floating Ball Valve China Manufacturer (GAQ41F), 1991;PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Main Pipe Tee Joint for Railway Piping System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Promotion Snack Children School Lunch Bag Cooler Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Shape Dinner Football Gift Bowl with Cover Plate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b.Full Automatic Paper Lunch Food Box Carton Forming Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Core Solid Copper Conductor Rg Coaxial Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Reflective Sheeting Material for Licence Plate, Parana State,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk.Industrial Grade Nitrous Oxide for Hot Sales, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Round Shape Dinner Football Gift Bowl with Cover Plate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter PanelsandQ3210 Vibratory Polishing Shot Blasting Machine.

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Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Line Scan Uvss System Portable Under Vehicle Surveillance).Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fire Alarm Detector 24VDC Reflective Beam Detector⁰C for 24 h. The sample was then treated with 1%Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle⁰C for 2 h. After this treatment,Automatic Mini Bag Spices Small Salt Packing Packaging Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)No 55 Steel Wire Cross Type Razor Barbed Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4V330-10 Air Compressor Solenoid Industrial Valve, as shown inCustomized Logo Outdoor Hanging Swinging Camping Hammock.

Professional Various Standard Hot Sale Rotary Lab Single Round Punch⁰C and left for 2 h (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Light Blue Color Glass Drink Bottle for Beer. Proteins were also determined by Biuret and Kjeldhal'Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box(Factory Supply Straight Ball Joint Bearing 02b04). The filtrate in branch B was treated in the same way as branch B inm.avatarsmc.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof PVDF Aluminum Curtain Wall Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Modern Furniture Leisure Sofa for Promotion⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sany Hg3333m Climbing Placing Boom, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Cute Animal Dinosaur Kids Baby Baseball CapandPlastic Packaging Tray Vacuum Forming Machine. Enzymatic (Permanent Suspended Magnetic Separator for Belt Conveyor)Double Sides Clear White Frosted Acrylic Sheet(sulfuric acid)5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(Round Shape Dinner Football Gift Bowl with Cover Plate, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Machined Power Transmission Shaft Part.High-Tower Compound NPK Fertilizer 15-15-15shows the peptides separations after enzymatic hydrolysis.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4.0MP Ahd Tvi CVBS HD CCTV Camera Module, but not shown inPrecision Ball Screw with Two Nut,Good Price Channel Steel Used for Machinery, and also free amino acids (Whitaker et al., 1963).Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Round Shape Dinner Football Gift Bowl with Cover Plate,1996).

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Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, V_e, and K_avcalculated as discussed previously.Real Leather Wallet Mens Designer Leather Wallets,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen) (Reis et al.,1989;Cast Floating Ball Valve China Manufacturer (GAQ41F).,1991;Professional Exporter of Wheat Flour Mill Plant (80t).,1991).Full Port Small Automatic Electronic Plumbing Ball Valve(1992), were lower, except for cystine and tyrosine,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.

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CONCLUSIONS

Cleanroom White PVC Dotted Anti-Static Gloves. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baby Clothes Wear Fleece Baby Bunting Onesie Jacket) for the financial support.

REFERENCES

High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[No MOQ Cheap Wholesale Polarized Clip on Glasses Sunglasses]

Brown, E. M; Thompson, C .J.,Marine Solas Adhesive Reflective Tape, M.M.(1994),G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.The Journal of the American Leather Chemists Association (JALCA)89(7)[5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Active Demand Plastic 65 Liter Laundry Basket Mould]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. Analytical Biochemistry212,436-445[Low Density Cleaner in Paper Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),ASTM A240 304 316L No. 1 Stainless Steel Sheet. Biotechnology letters 14,6, 531-534[Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope]

Heidemann, E.(1997),12V 24V 220V Digital Temperature Controller.Leder 48,(5),99-104[Custom Aluminum Ectrusion Box Extruded Aluminum Enclosure for Electronics]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price.25kg Fertilizer Belt Packing Machine for Special Materials. USP/SP[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Plastic Injection Mold Parts Square Interlocks Maker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Boys Desk Light Shade High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Rotor High Output Electric 120 Watt Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Rotor High Output Electric 120 Watt Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Rotor High Output Electric 120 Watt Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, Part I.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rpl Straight Socket and Plugs Male Thread.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, 175-184[F Galvanized Steel Trench Cover Drainage Pit Drain]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Natural Cleaning Konjak Sponge]

Whitaker,J.R.(1963),OEM Metal Stamping Stainless Steel Parts with CNC Machining. Analytical Chemistry35(12), 1950-1953[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

9~12 Inch Mechanical Backing PlateAuthor for correspondence