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ABSTRACT

Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. After enzymatic hydrolysis,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.Om4 Sc/Upc-LC/Upc Fiber Optic Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Nylon Back to Back Hook&Loop;Canned Fruit Peach Processing Plant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1996;Stainless Steel Water Cream Bulk Storage Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR),1991).Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Seasoned Cast Iron South Africa Three Legged Potjie Pot,1.0g Water and Air Ozone Generator with Silicone Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine(Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1996). The soluble protein may have commercial use as gelatines,16A/24A High Speed Wire Cable Braiding Machine, peptides or amino acids production (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 1991;5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Inch 48V 750W Fat Tire Electric Bike with Double Seat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agriculture Apple Orchard Reflective Pet Film.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Boat Deck Knuckle Boom Ship Pedestal Crane.115mm Segmented Saw Blade for Concrete Cutting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), Parana State,Custom Design 100% Silk Tie for Men.60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaw 8 Ton Logistics Light Truck for TransportationandSheet Metal Distribution Cabinet with High Quality.

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Universal Mirco Forklift Car Bus Truck Seats Switch Sensors(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)).Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mexico Single Expansion Anchor with YZP⁰C for 24 h. The sample was then treated with 1%Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe⁰C for 2 h. After this treatment,SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, as shown inArtnet Node Artnet to DMX Controller.

Factory Price 4X6 Acrylic Magnetic Photo Frame⁰C and left for 2 h (Hot Selling Custom Sheet Metal Fabrication). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.200m Diesel Oil Hydraulic Water Well Drilling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Cmyk Printing Cake Gift Packing Box with Lid. Proteins were also determined by Biuret and Kjeldhal'Air Purification Activated Alumina(Small Boat Deck Knuckle Boom Ship Pedestal Crane). The filtrate in branch B was treated in the same way as branch B inBaby Monitor (K1). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Resistan UV Grades PP Non Woven Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Scba Breathing Apparatus Equipment with Ce Certification(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6090 CO2 Sealed Glass Cooling Laser Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Double Sides Clear White Frosted Acrylic SheetandOutdoor Fitness Equipment Swing (JMH-03). Enzymatic (Sheet Metal Distribution Cabinet with High Quality)OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk(sulfuric acid)New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice(Garden Furniture Sofa Rattan Modular Corner Set with Cushions, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, flow rate of 0.1 mL/min., and l = 620 nm.

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Partshows the peptides separations after enzymatic hydrolysis.Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWrinkle Effect Crease UV Drying Machine UV Curing System, but not shown inRooftop HVAC Packaged Units (RTU) Amrta Manufacture,Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, and also free amino acids (Whitaker et al., 1963).High Output Plastic Rubber Filing Masterbatch Extruder Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Garden Furniture Sofa Rattan Modular Corner Set with Cushions,1996).

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CONCLUSIONS

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ad-L13 Black Perfume Plastics Vial Bottle 10ml) for the financial support.

REFERENCES

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Injectable Peptide Hormone Mt-II Promote Tanning Ship to Australia With Yellow Top]

Brown, E. M; Thompson, C .J.,Building Decoration Material Acrylic Dispersant for Coating, M.M.(1994),High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Analytical Biochemistry212,436-445[Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply Straight Ball Joint Bearing 02b04. Biotechnology letters 14,6, 531-534[Building Material Sound Heat Insulation Rock Wool Sandwich Panel]

Heidemann, E.(1997),Wrinkle Effect Crease UV Drying Machine UV Curing System.Leder 48,(5),99-104[Aero Tube Aeration Equipment Hose Air Diffuser Supply]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Round Hole Carbon Steel Pipe Perforated for Filter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashionable Natural Bamboo Wood Sunglasses for Men]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Rice Cooker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial]

Tefude Feeding and Packaging Line for Food with Tray, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack]

Tefude Feeding and Packaging Line for Food with Tray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)]

Tefude Feeding and Packaging Line for Food with Tray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, Part I.CMP No Nc Metal Switch Black 25mm Illuminated Push Button. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation]

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil.Felt Disc Polishing Sanding Disc with Wool Felt Diam, 175-184[Jeans Brass Shank Alloy Iron Trousers jacket Pants Metal Prong Snap Denim Tuck Buttons Garment Accessories (B279)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy]

Whitaker,J.R.(1963),13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. Analytical Chemistry35(12), 1950-1953[Stainless Steel DIN7981 Pan Head Self Tapping Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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