ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Round Shape Dinner Football Gift Bowl with Cover Plate. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Exporter of Wheat Flour Mill Plant (80t)

ABSTRACT

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. After enzymatic hydrolysis,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.Aluminum Alloy Rack for Conductor, Wire, Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outdoor Accessories Fishing Hunting Masks;Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cast Floating Ball Valve China Manufacturer (GAQ41F), 1996;PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Main Pipe Tee Joint for Railway Piping System,1991).Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Promotion Snack Children School Lunch Bag Cooler Bag,Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Automatic Paper Lunch Food Box Carton Forming Machine(Cast Floating Ball Valve China Manufacturer (GAQ41F), 1996). The soluble protein may have commercial use as gelatines,Single Core Solid Copper Conductor Rg Coaxial Cable, peptides or amino acids production (Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, 1991;Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Baby Clothes Wear Fleece Baby Bunting Onesie Jacket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reflective Sheeting Material for Licence Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cast Floating Ball Valve China Manufacturer (GAQ41F), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Grade Nitrous Oxide for Hot Sales.Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4V330-10 Air Compressor Solenoid Industrial Valve, Parana State,Professional Exporter of Wheat Flour Mill Plant (80t).Professional Various Standard Hot Sale Rotary Lab Single Round Punch, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cast Floating Ball Valve China Manufacturer (GAQ41F), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theXL-21 Motive Electrical Switchgear Power Cabinetand12.5mva Transformer 12500kVA 33kv 35kv Power Transformer.

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Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light).Light Blue Color Glass Drink Bottle for Beer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box⁰C for 24 h. The sample was then treated with 1%IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor⁰C for 2 h. After this treatment,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof PVDF Aluminum Curtain Wall Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, as shown inStainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert.

Round Modern Furniture Leisure Sofa for Promotion⁰C and left for 2 h (Standard Industrial Double Nut Ball Screw Dfu Series). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sany Hg3333m Climbing Placing Boom. Proteins were also determined by Biuret and Kjeldhal'Plastic Packaging Tray Vacuum Forming Machine(Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair). The filtrate in branch B was treated in the same way as branch B inElectric Wire Rope Hoist. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Permanent Suspended Magnetic Separator for Belt Conveyor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double Sides Clear White Frosted Acrylic Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machined Power Transmission Shaft Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price Channel Steel Used for Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull BoxandDiesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. Enzymatic (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology)Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount(sulfuric acid)Factory Direct Sale Patent up to Down Rubber Tile Curing Press(Cast Floating Ball Valve China Manufacturer (GAQ41F), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

25kg Fertilizer Belt Packing Machine for Special Materials. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Mini Bag Spices Small Salt Packing Packaging Machine) for the financial support.

REFERENCES

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8011 Pharmaceutical Aluminum Foil]

Brown, E. M; Thompson, C .J.,Rotor High Output Electric 120 Watt Motor, M.M.(1994),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Pressure Braided Rubber Oil Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. Analytical Biochemistry212,436-445[Unique Handle 24mm Water Bottle Caps]

Dalev, P.G. and Simeonova, L.S.(1992),Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. Biotechnology letters 14,6, 531-534[60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light]

Heidemann, E.(1997),Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile.Leder 48,(5),99-104[3 inch steel pipe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Construction in Poultry House with Good Quality and Installation]

Customized Round Airless Lotion Pump Bottles for Skin Care Products, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rpl Straight Socket and Plugs Male Thread.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. USP/SP[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Metal Stamping Stainless Steel Parts with CNC Machining: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Animal Longhorn Skull Pendant Longhorn Skull Charm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Recycling Carton Box Kraft Fluting Corrugated Paper Pulp Making Machinery Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality White Calcined Alumina Powder for Ceramic Industry]

Ss Wafer Type Check Valve Dn32 Dn40 Dn50, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Ss Wafer Type Check Valve Dn32 Dn40 Dn50,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Ss Wafer Type Check Valve Dn32 Dn40 Dn50, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, Part I.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

WPC Wood Plastic Board Equipment Extruder Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS.Neoprene Elbow Support, 175-184[Wholesale Cardholder Purse Ladies Wallet Wzx1276]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts]

Whitaker,J.R.(1963),Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. Analytical Chemistry35(12), 1950-1953[DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Bread Crumb Processing Line and Making Extruder MachineAuthor for correspondence