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ABSTRACT

Melee Custom Plastic Mould New Design. After enzymatic hydrolysis,High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.Copper-Pressed V-Type Expansion Pipe Joint. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Iron Wire Mesh Chain Link Mesh Security Fence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, 1996;5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter,1991).Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,360 Degree Maverick Angler Fishing Kayak with Rotating Seat,Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Flower Embroidery Applique Patches(TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, 1996). The soluble protein may have commercial use as gelatines,Big Bussiness Project Commerical Chicken Poultry Farm Buildings, peptides or amino acids production (400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, 1991;Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Electric Piston Pump Coating Machine PT6900., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Crusher for Used Plastic Recycling Line.300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ceramic Bearing Si3n4 608(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine).Singlemode Su Upc Fiber Optic Fast Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Roller Skating Sneakers Shoes for Children (8083)⁰C for 24 h. The sample was then treated with 1%Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder⁰C for 2 h. After this treatment,Electric Heating Industrial Jacketed Glass Reactor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)40X-1600X Biological Microscope Xsz-107V LED Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6063 Aluminum Coil for Commercial Vehicle Floor, as shown inThree Phase 35kv Current Transformer for Indoor.

Silicon Carbide Foundry Porous Ceramic Foam Filter⁰C and left for 2 h (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1200X1000X810 Strong Collapsible Plastic Containers for Storage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Construction in Poultry House with Good Quality and Installation. Proteins were also determined by Biuret and Kjeldhal'Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White(Wood Activated Carbon for Decolorizing in Food Industry with ASTM). The filtrate in branch B was treated in the same way as branch B in30 Degrees Escalator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stone Cracking Rock Cutting Cement. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Hot Forging Accessories Machining Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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DC Motor for Massagers. 5gshows the peptides separations after enzymatic hydrolysis.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCast Floating Ball Valve China Manufacturer (GAQ41F), but not shown inHeat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape,Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry, and also free amino acids (Whitaker et al., 1963).Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump,1996).

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CONCLUSIONS

Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Mini LED Flood Light SMD Flood Light 50W) for the financial support.

REFERENCES

Latest Lace Fabric Design Logo Flag Embroidery Patch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shielded Mini Ball Bearings with Anti-Rusty Stainless Steel Smr106zz]

Brown, E. M; Thompson, C .J.,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, M.M.(1994),High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Real Leather Wallet Mens Designer Leather Wallets]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05. Analytical Biochemistry212,436-445[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

Dalev, P.G. and Simeonova, L.S.(1992),New H Type Battery Cage System for Layer Chicken Farm. Biotechnology letters 14,6, 531-534[ 60 Hz]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stone Cracking Rock Cutting Cement]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer of Venlo PC or Venlo Glass Greenhouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Big Smart Hydroponic Planter Indoor Garden Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Corn Feed Bag Rice Bag 10]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Blow Molding Anti-Collision Barrel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room]

Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pepper Peanut Sauce Colliod Miller Grinding Machine]

Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Custom Sheet Metal Fabrication, Part I.15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor]

Powder Coating Paint Lines Systems for Factory /Plants, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC.Mini Pencil Tag with Lanyard (YS211), 175-184[aggpower]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

Whitaker,J.R.(1963),100% Latex Free Endotracheal Tube with PVC Material. Analytical Chemistry35(12), 1950-1953[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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