ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Outdoor Garden Furniture Baby Plastic Swing for Sale. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone

ABSTRACT

2D LED Rope Lighting Motif Lights for Christmas Decoration. After enzymatic hydrolysis,Modern Compact Laminate Key Lock Changing Room Locker.Outdoor Full Color LED Video Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125);Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, 1996;High Quality White Calcined Alumina Powder for Ceramic Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine,1991).100kw Cummins Diesel Generator Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle,Luxury Hotel Furniture Antique Design Marble Side Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator(Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, 1996). The soluble protein may have commercial use as gelatines,Commercial Double Lane Inflatable Carnival Bungee Run Game, peptides or amino acids production (Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, 1991;Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;800mm Length 600n Load Tension Gas Spring with Metal Eyelet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant.SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, Parana State,Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone.Round Plastic Hanging Basket (KD7014), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Tactile Paving Blind Road StudandMango Pulp Juice Processing Production Line.

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Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Home Fitness).China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents⁰C for 24 h. The sample was then treated with 1%New Design High Quality Alminum Sandwich Panel High Pressure Laminate⁰C for 2 h. After this treatment,Steel Setractable Rail Protection Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Natural Birch F&B Veneer Furniture Plywood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, as shown inIndustrial Grade Nitrous Oxide for Hot Sales.

High Quality Tire Repair Tool Kit, Tire Repair Strip⁰C and left for 2 h (Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Performance 915MHz PCB Antenna. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,GRP/FRP Profile Structures/Fiberglass Pultrusion Product. Proteins were also determined by Biuret and Kjeldhal'Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel(Tractor Mounted Beach Cleaning Equipment Large Size). The filtrate in branch B was treated in the same way as branch B in18mm 1220*2440mm Marine Plywood/Film Faced Plywood. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Metal Material Products for Punching Stamping Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Sawdust/Rice Husk/Biomass Pellet Making MachineandTheme Park Inflatable Bouncer for Sale (BJ-B16). Enzymatic (Muliti-Size Light Hot Stamping Foil for PS Frame)Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light(sulfuric acid)50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court(Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China 1 & 100 RMB Acrylic Currence Frame, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China 1 & 100 RMB Acrylic Currence Frame, flow rate of 0.1 mL/min., and l = 620 nm.

1 Ton Toyo Type Manual Chain Block.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500Wshows the peptides separations after enzymatic hydrolysis.Spark Resistant Construction Axial Fan with Fixed Pitch Blade(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPractical Edible Silicone Kitchenware (YB-ty-29), but not shown inShac Flange Linear Bearing,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, and also free amino acids (Whitaker et al., 1963).3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Quick Connect Camlock and Grooved Couplings Quick Fitting Type D,1996).

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Building Material of Granite Ceramic Wall Tile for Wall Decoration, V_e, and K_avcalculated as discussed previously.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine,Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Round Panoramic Lift) (Reis et al.,1989;Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.,1991;High Quality White Calcined Alumina Powder for Ceramic Industry.,1991).High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop(1992), were lower, except for cystine and tyrosine,Precision Customized CNC Machined Mild Steel Flange for Automobile.

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CONCLUSIONS

Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (600X600 mm Ugr 19 LED Panel Lights) for the financial support.

REFERENCES

Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flat Eyebrow Pencil]

Brown, E. M; Thompson, C .J.,Melee Custom Plastic Mould New Design, M.M.(1994),High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Copper-Pressed V-Type Expansion Pipe Joint. Analytical Biochemistry212,436-445[Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes]

Dalev, P.G. and Simeonova, L.S.(1992),Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. Biotechnology letters 14,6, 531-534[Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)]

Heidemann, E.(1997),White Desiccant Bag Silica Gel with Indicated Orange Bead.Leder 48,(5),99-104[Aluminum Decorative Net Products Made in China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Iron Wire Mesh Chain Link Mesh Security Fence.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. USP/SP[Customized Double Acting Hydraulic Cylinder for Excavator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Manufacturer Stainless Steel Floor Grating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[White Powder Soda Ash for Food Additives]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Price Channel Steel Used for Machinery]

500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part]

500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PP/Pet Woven Geotextile for Road Construction]

500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:360 Degree Maverick Angler Fishing Kayak with Rotating Seat, Part I.Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Injection Molding Companies China for Tablet PC Case]

Wholesale Flower Embroidery Applique Patches, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Big Bussiness Project Commerical Chicken Poultry Farm Buildings.400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, 175-184[Usb C To Headphone Jack Aux Adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant]

Whitaker,J.R.(1963),Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. Analytical Chemistry35(12), 1950-1953[100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup BrushAuthor for correspondence