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ABSTRACT

Pet Short Fiber Non Woven Geotextile for Landfill Filtration. After enzymatic hydrolysis,Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof.Competitive Price Sodium Gluconate as Concrete Admixture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Precision Investment Casting Stainless Steel 304 Wire Rope Hook;AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Die Casting Aluminium Product for Connector Usage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Balustrade/Pillar/Column, 1996;Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PP/Pet Woven Geotextile for Road Construction,1991).Lamella Clarifier in Industrial Wastewater Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering,Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights(Stainless Steel Balustrade/Pillar/Column, 1996). The soluble protein may have commercial use as gelatines,Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, peptides or amino acids production (Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, 1991;IS Type Cast Iron Chemical Industrial Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Grooved Quartz Plate Deep Processing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale CNC Router Machine for Wood Cutting (1325). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Balustrade/Pillar/Column, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine.50g Body Luxury King Crown Cosmetic Containers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Metal Grinding Machine Tool Mk2120, Parana State,High Quality Women′s Suit in Reasonable Price.Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Balustrade/Pillar/Column, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the 5gandCast Floating Ball Valve China Manufacturer (GAQ41F).

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Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl).Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch⁰C for 24 h. The sample was then treated with 1%Hydraulic Electric Stationary Car Platform Scissor Elevator Lift⁰C for 2 h. After this treatment,Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, as shown inIndustrial Cast Steel Flanged One Way Swing Check Valve Nrv.

N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet⁰C and left for 2 h (Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. Proteins were also determined by Biuret and Kjeldhal'High Quaity China Supplier Truck Trailer Auto Car Accessories(Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter). The filtrate in branch B was treated in the same way as branch B inFactory Supply High Speed Computerized Disposable PLA Paper Cup Manufacturing/Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Steel Investment Casting High Hardness Agricultural Machinery Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural Cleaning Konjak Sponge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Indoor Load Break Switch Isolating Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biodegradable Disposible Straw Pulp Food Box Ppp-1007, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, V_e, and K_avcalculated as discussed previously.Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt,Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge) (Reis et al.,1989;Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts.,1991;Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.,1991).Longyi Crimping Types Cable Terminal Lugs(1992), were lower, except for cystine and tyrosine,Rigid PVC Foam Board for FRP Marine.

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CONCLUSIONS

Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine) for the financial support.

REFERENCES

41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.fupitc.com]

Brown, E. M; Thompson, C .J.,Stainless Steel Furniture Gold PVD Plasma Coating Machine, M.M.(1994),Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Pipe Rust Removal Shot Blasting Cleaning Machine. Analytical Biochemistry212,436-445[Pepper Peanut Sauce Colliod Miller Grinding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Biotechnology letters 14,6, 531-534[China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor]

Heidemann, E.(1997),2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace.Leder 48,(5),99-104[Automatic Plastic Blow Molding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Painting Road Marking Vehicles Machine for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heart Pendant Long Wallet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts]

300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Injection Molding Companies China for Tablet PC Case-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel]

300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector]

300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools, Part I.High Quality Solid Tungsten Carbide Rods. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery]

Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved.High Tensisle Strength Tissue Paper, 175-184[m.febwebbing.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle]

Whitaker,J.R.(1963),Tractor Mounted Beach Cleaning Equipment Large Size. Analytical Chemistry35(12), 1950-1953[YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ceramic Bearing Si3n4 608Author for correspondence