ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder

ABSTRACT

Mexico Single Expansion Anchor with YZP. After enzymatic hydrolysis,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope;Whole Absorbtion Flexo Printing Soltting Die Cutting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price 4X6 Acrylic Magnetic Photo Frame, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (200m Diesel Oil Hydraulic Water Well Drilling Machine, 1996;Custom Cmyk Printing Cake Gift Packing Box with Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Air Purification Activated Alumina,1991).Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Resistan UV Grades PP Non Woven Fabric,New Scba Breathing Apparatus Equipment with Ce Certification,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FRP Pultruded Pipe or GRP Profile(200m Diesel Oil Hydraulic Water Well Drilling Machine, 1996). The soluble protein may have commercial use as gelatines,6090 CO2 Sealed Glass Cooling Laser Cutting Machine, peptides or amino acids production (Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, 1991;Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Fitness Equipment Swing (JMH-03)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sheet Metal Distribution Cabinet with High Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (200m Diesel Oil Hydraulic Water Well Drilling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice.Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Animal Longhorn Skull Pendant Longhorn Skull Charm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+PantAlcalase2.5 L DX,Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Output Plastic Rubber Filing Masterbatch Extruder Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Precision Mold Manufacturer Plastic Injection Mould(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

China Wholesale Oxide Aluminium Black Sand Blast Media, Parana State,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.Welded Stud Anchor Chain for Ship, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (200m Diesel Oil Hydraulic Water Well Drilling Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theC Purlin/C Steel Beam/ C Section Steel/ C Shaped SteelandRapid Prototyping for Aluminum Parts Sometimes Steel or Brass.

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SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ceramic Tile Plasma Ion Deposition Planting Functional PVD Vacuum Coating Machine).Line Scan Uvss System Portable Under Vehicle Surveillance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Folding Steel Manual Wheelchair with Chrome Frame⁰C for 24 h. The sample was then treated with 1%Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel⁰C for 2 h. After this treatment,Fashion Style Lady Alloy Quartz Watch with Simple Dial. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rooftop HVAC Packaged Units (RTU) Amrta Manufacture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, as shown inPrecision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.

Building Decoration Material Acrylic Dispersant for Coating⁰C and left for 2 h (Round Plastic Hanging Basket (KD7014)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Proteins were also determined by Biuret and Kjeldhal'Factory Supply Straight Ball Joint Bearing 02b04(K Helical Bevel Gearboxes Reducer Motor). The filtrate in branch B was treated in the same way as branch B in40 FT Container Transport Skeleton Semi Truck Trailer Chassis for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wrinkle Effect Crease UV Drying Machine UV Curing System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Pinpointer Metal Detector Hand Held Underground Gold Metal Detector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tefude Feeding and Packaging Line for Food with Tray⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Gl-321t IP Network Sound System Ceiling Speaker Indoor PA SpeakersandCMP No Nc Metal Switch Black 25mm Illuminated Push Button. Enzymatic (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks)Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(sulfuric acid)Felt Disc Polishing Sanding Disc with Wool Felt Diam(200m Diesel Oil Hydraulic Water Well Drilling Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System, flow rate of 0.1 mL/min., and l = 620 nm.

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable.High Quality Nylon Back to Back Hook&Loopshows the peptides separations after enzymatic hydrolysis.100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, but not shown inDC 12V 24V Mini Portable Car Refrigerator Compressor Fridge,240T goodeng water/oil/gas pipe drilling rig HDD machine, and also free amino acids (Whitaker et al., 1963).Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (200m Diesel Oil Hydraulic Water Well Drilling Machine,1996).

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Universal Wholesale Hot Sale Custom Blank Keys, V_e, and K_avcalculated as discussed previously.Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge,Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale) (Reis et al.,1989;Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator.,1991;Custom Cmyk Printing Cake Gift Packing Box with Lid.,1991).Waw 8 Ton Logistics Light Truck for Transportation(1992), were lower, except for cystine and tyrosine,Hot Forging Lathe Spindle Forged Spindle.

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CONCLUSIONS

Hot Sale Chic Straw Braid Floppy Hat with Print Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Output Plastic Rubber Filing Masterbatch Extruder Machine) for the financial support.

REFERENCES

High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10R 280W Beam]

Brown, E. M; Thompson, C .J.,Lamella Clarifier in Sewage Tretament Plant for Water Purification, M.M.(1994),BMC (DMC) / SMC Compression Moulding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hydraulic Electric Stationary Car Platform Scissor Elevator Lift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ring Caps &Plastic Tube Pop Novelties. Analytical Biochemistry212,436-445[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. Biotechnology letters 14,6, 531-534[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Heidemann, E.(1997),Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk.Leder 48,(5),99-104[Factory Direct Supply Sterile Removal Wrinkle 100% Pure Hyaluronic Acid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fire Alarm Detector 24VDC Reflective Beam Detector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Xtsky High Quatily Good Price Solar Lamp Air Filter 96591485]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Low Density Cleaner in Paper Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Design Luxury Moon Cake Box with Hot Stamp Logo]

Low Density Cleaner in Paper Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Minimalist Slim Real Leather Front Pocket Card Wallet]

Low Density Cleaner in Paper Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Stainless Steel Laser Cutting, Part I.Price of a Retail Supermarket Push Cart for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China 1 & 100 RMB Acrylic Currence Frame]

Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate.Handmade Natural Bamboo Basket / Bamboo Flower Pot, 175-184[Carbide Burr Cutter Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Air Purification Activated Alumina]

Whitaker,J.R.(1963),Stainless Steel Handrail Bar Holder for 16mm Pipe. Analytical Chemistry35(12), 1950-1953[100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sany Hg3333m Climbing Placing BoomAuthor for correspondence