ARTICLES

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ABSTRACT

Fire Alarm Detector 24VDC Reflective Beam Detector. After enzymatic hydrolysis,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle.Automatic Mini Bag Spices Small Salt Packing Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Various Standard Hot Sale Rotary Lab Single Round Punch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, 1996;Light Blue Color Glass Drink Bottle for Beer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box,1991).IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine,Waterproof PVDF Aluminum Curtain Wall Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas(Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, 1996). The soluble protein may have commercial use as gelatines,Round Modern Furniture Leisure Sofa for Promotion, peptides or amino acids production (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, 1991;Sany Hg3333m Climbing Placing Boom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Packaging Tray Vacuum Forming Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Permanent Suspended Magnetic Separator for Belt Conveyor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Sides Clear White Frosted Acrylic Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machined Power Transmission Shaft Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Bulb Light UFO Light Recruitment Distributor).Real Leather Wallet Mens Designer Leather Wallets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)⁰C for 24 h. The sample was then treated with 1%Full Port Small Automatic Electronic Plumbing Ball Valve⁰C for 2 h. After this treatment,Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cleanroom White PVC Dotted Anti-Static Gloves's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, as shown inHigh Quality Women′s Suit in Reasonable Price.

Marine Solas Adhesive Reflective Tape⁰C and left for 2 h (Hsac Shac Linear Guideway). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. Proteins were also determined by Biuret and Kjeldhal'ASTM A240 304 316L No. 1 Stainless Steel Sheet(FRP Pultruded Pipe or GRP Profile). The filtrate in branch B was treated in the same way as branch B inFactory Price Raw Coal Anthracite Filter with High Quality. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12V 24V 220V Digital Temperature Controller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av25kg Fertilizer Belt Packing Machine for Special Materials(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rotor High Output Electric 120 Watt Motor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, V_e, and K_avcalculated as discussed previously.CNC Produce Medical Machining Custom Metal Milling,Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl) (Reis et al.,1989;Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket.,1991;Light Blue Color Glass Drink Bottle for Beer.,1991).Custom Metal Stamping Welding Spare Parts for Farming(1992), were lower, except for cystine and tyrosine,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

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CONCLUSIONS

Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator) for the financial support.

REFERENCES

275ml Frosted Clear Glass Drinking Bottle for Juice, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Popular Paper Food Box Forming Machine]

Brown, E. M; Thompson, C .J.,EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price, M.M.(1994),Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bolts and Nuts Set Brass Fasteners Double Hex Bolt. Analytical Biochemistry212,436-445[High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Sales Kids Boy Cotton Children T Shirt. Biotechnology letters 14,6, 531-534[SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet]

Heidemann, E.(1997),Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.Leder 48,(5),99-104[Implantology Instruments]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fully Automatic Water Bottle Lids Labeling Machine]

Industrial Grade Nitrous Oxide for Hot Sales, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi.Light Blue PVC Plastic Sheet and PVC Film. USP/SP[Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Screw/Countersunk Head Screw 6 Ribs with Wing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[HPMC Hydroxy Propyl Methyl Cellulose Additives]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Building Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology]

Metric Female Cone Hydraulic Hose Fitting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Dental Material Dental PMMA Block From China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paper Egg Box Forming Molding Production Line/ Egg Tray Machine]

Metric Female Cone Hydraulic Hose Fitting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope]

Metric Female Cone Hydraulic Hose Fitting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Waste Pet Flakes Recycling Washing Machine, Part I.Deposited Stainless Steel Rivet Nut with Knurled. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card]

High Quality Stainless Steel Mechanical Filter Hosing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Paper New Year Greeting Cards Wedding Invitations Cards.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), 175-184[Fabric Barstool]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metric Female Cone Hydraulic Hose Fitting]

Whitaker,J.R.(1963),BLE5.0 Ttl to Bluetooth Module with at Command. Analytical Chemistry35(12), 1950-1953[Green Color PVC Anti Dazzle Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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