ARTICLES

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ABSTRACT

Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. After enzymatic hydrolysis,Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer.500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1996;Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Longyi Crimping Types Cable Terminal Lugs,1991).Rigid PVC Foam Board for FRP Marine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Furniture Gold PVD Plasma Coating Machine(Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, peptides or amino acids production (Steel Pipe Rust Removal Shot Blasting Cleaning Machine, 1991;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Bread Crumb Processing Line and Making Extruder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring.300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Injection Molding Companies China for Tablet PC Case(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Tensisle Strength Tissue Paper, Parana State,Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw.Tractor Mounted Beach Cleaning Equipment Large Size, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the27PCS Oscillating Multi Tool Blades Set for Multi-Purpose ToolsandSteel Floor Deck Post Cutting Cold Roll Forming Machine.

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100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture).Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Coated Pipe Machine Extruder Machine⁰C for 24 h. The sample was then treated with 1%China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts⁰C for 2 h. After this treatment,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mining Machinery Parts Diamond Saw Blade Segments for Marble's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, as shown inEAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).

Multi Column Load Cell 100kg, 20t⁰C and left for 2 h (Stainless Steel Water Cream Bulk Storage Tank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. Proteins were also determined by Biuret and Kjeldhal'Foldable PP Plastic Storage Logistics Turnover Box(Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building). The filtrate in branch B was treated in the same way as branch B inAir And Water Splice Coupler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Buddhist Mood Picture Wallpaper Murals. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Flange Dismantling Joint(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Precision castings

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CONCLUSIONS

Custom Free Logo Design Cheap Hand Flag (NF01F02021). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover) for the financial support.

REFERENCES

125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medical Diagnostic Dengue Igg/Igm Test Kit]

Brown, E. M; Thompson, C .J.,Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, M.M.(1994),RJ45 Cat5e in-Line Coupler Modular Jack.The Journal of the American Leather Chemists Association (JALCA)89(7)[Handmade Natural Bamboo Basket / Bamboo Flower Pot]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Hot Rolled Steel U Channel China Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. Analytical Biochemistry212,436-445[Powder Coating Paint Lines Systems for Factory /Plants]

Dalev, P.G. and Simeonova, L.S.(1992),Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. Biotechnology letters 14,6, 531-534[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

Heidemann, E.(1997),Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052.Leder 48,(5),99-104[Contact Smart Card Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Paper New Year Greeting Cards Wedding Invitations Cards]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Heating Industrial Jacketed Glass Reactor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brass Insert Ppr Pipe Fittings High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

High Quality Brushless DC Electric Motor for Industrial Deduster, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Factory Light Soda Ash /Sodium Carbonate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

High Quality Brushless DC Electric Motor for Industrial Deduster,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM ABS Industrial Plastic Sheet Injection Auto Part]

High Quality Brushless DC Electric Motor for Industrial Deduster, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Regular Size Cigarette Rolling Papers, Part I.304L Stainless Steel U Channel Good Performance. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Junior Backyard Training Practise Soccer Goal Net.350mm Diamond Blade for Granite, 175-184[Brown Glass Beer Bottle With Crown Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Kitchen Floor Mat]

Whitaker,J.R.(1963),Custom Welding Stainless Steel Sheet Metal Welding Parts. Analytical Chemistry35(12), 1950-1953[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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