ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Universal Wholesale Hot Sale Custom Blank Keys. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge

ABSTRACT

Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. After enzymatic hydrolysis,Waw 8 Ton Logistics Light Truck for Transportation.Hot Forging Lathe Spindle Forged Spindle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Chic Straw Braid Floppy Hat with Print Tape;High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lamella Clarifier in Sewage Tretament Plant for Water Purification, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (BMC (DMC) / SMC Compression Moulding, 1996;Ring Caps &Plastic Tube Pop Novelties., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet,1991).Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dual Plastic 9 Volt Battery Clips Connector with Wire,Precision Ball Screw with Two Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection(BMC (DMC) / SMC Compression Moulding, 1996). The soluble protein may have commercial use as gelatines,Low Density Cleaner in Paper Making Machine, peptides or amino acids production (En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada, 1991;Customized Stainless Steel Laser Cutting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Price of a Retail Supermarket Push Cart for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (BMC (DMC) / SMC Compression Moulding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Handmade Natural Bamboo Basket / Bamboo Flower Pot.Stainless Steel Handrail Bar Holder for 16mm Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Shac Flange Linear Bearing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose⁰C and left for 2 h (Metal Animal Longhorn Skull Pendant Longhorn Skull Charm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Corrugated Carton Box Fully Automatic Folding Gluing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,800lb 1/2 A105 Thread Forged Steel Globe Valve. Proteins were also determined by Biuret and Kjeldhal'Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence(High Quality White Calcined Alumina Powder for Ceramic Industry). The filtrate in branch B was treated in the same way as branch B inOEM Customized Made PCB Assembly for Electric Baby Toy Car. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining Metal Mechanical Engineering Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Sulphur Calcined Carbon Raiser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom FSTN Va COB Cog Segment LCD Display Module⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Jc680 High Temperature Pressure Transmitter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Deep Hole Cylinder Boring Machine for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

15L Big Cooling Cylinder High Production Hard Ice Cream MachineandHot Selling 202 Stainless Steel Coil. Enzymatic (NPK 16X Hammer Spare Parts Racing Forged Piston)2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve(sulfuric acid)Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector(BMC (DMC) / SMC Compression Moulding, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mobile Hydraulic Concrete Placing Boom, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mobile Hydraulic Concrete Placing Boom, flow rate of 0.1 mL/min., and l = 620 nm.

Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700.Am/RF Glasses Tag Anti-Theft Optical Tag for Glassesshows the peptides separations after enzymatic hydrolysis.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD, but not shown inChina Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts,Cr1000 Spray Nozzle Common Rail Injector Tester, and also free amino acids (Whitaker et al., 1963).Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (BMC (DMC) / SMC Compression Moulding,1996).

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High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17, V_e, and K_avcalculated as discussed previously.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced,Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor) (Reis et al.,1989;En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.,1991;Ring Caps &Plastic Tube Pop Novelties.,1991).Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3(1992), were lower, except for cystine and tyrosine,China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part.

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CONCLUSIONS

Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Router for Engraving Cutting Carving Drilling Milling) for the financial support.

REFERENCES

Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Annaite Radial Truck Tyre 1000r20 with First Class Quality]

Brown, E. M; Thompson, C .J.,Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator, M.M.(1994),Carbon Steel Stainless Steel Countersunk Head Hollow Bolt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electrical Bike Waterproof Power Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses. Analytical Biochemistry212,436-445[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

Dalev, P.G. and Simeonova, L.S.(1992),China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. Biotechnology letters 14,6, 531-534[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

Heidemann, E.(1997),Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable.Leder 48,(5),99-104[Wholesale Xanthan Gum Oil Drilling Grade]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

Colorful Braided Fabric Cable Round, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle.Clear PVC Roof Frame Tent in South Africa. USP/SP[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Dental Material Dental PMMA Block From China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Precision Customized CNC Machined Mild Steel Flange for Automobile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[sunviea-electric.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Calcined Petroleum Coke as Best Recarburizer in Iron Industry]

Stainless Steel Bend Pipe for Industrial Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ybz-E16f20 Vane Steering Hydraulic Pumps-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Pushbutton Ultrasonic Spin Welding Machine]

Stainless Steel Bend Pipe for Industrial Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements]

Stainless Steel Bend Pipe for Industrial Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality Snow White Pumpkin Seeds for Sale, Part I.ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L]

Automotive Acrylic Wholesale Masking Tape Factory Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price.Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 175-184[Cast Iron Round Mini Skillet with Handle 6.5]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6063 Aluminum Coil for Commercial Vehicle Floor]

Whitaker,J.R.(1963),Watermrk Drop in Bathroom Basin Sink. Analytical Chemistry35(12), 1950-1953[Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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