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ABSTRACT

White Desiccant Bag Silica Gel with Indicated Orange Bead. After enzymatic hydrolysis,High Quality Iron Wire Mesh Chain Link Mesh Security Fence.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (360 Degree Maverick Angler Fishing Kayak with Rotating Seat, 1996;Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Flower Embroidery Applique Patches,1991).Big Bussiness Project Commerical Chicken Poultry Farm Buildings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making,Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Electric Piston Pump Coating Machine PT6900(360 Degree Maverick Angler Fishing Kayak with Rotating Seat, 1996). The soluble protein may have commercial use as gelatines,200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, peptides or amino acids production (Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, 1991;High Quality Crusher for Used Plastic Recycling Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ceramic Bearing Si3n4 608.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nbm High Quality Auto Part Factory Price 6pk2310. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (360 Degree Maverick Angler Fishing Kayak with Rotating Seat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay.High Quality Mini LED Flood Light SMD Flood Light 50W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electric Heating Industrial Jacketed Glass Reactor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wood Fireplace Double Skin Chimney Flue Pipes).40X-1600X Biological Microscope Xsz-107V LED Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6063 Aluminum Coil for Commercial Vehicle Floor⁰C for 24 h. The sample was then treated with 1%Silicon Carbide Foundry Porous Ceramic Foam Filter⁰C for 2 h. After this treatment,1200X1000X810 Strong Collapsible Plastic Containers for Storage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Construction in Poultry House with Good Quality and Installation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, as shown in12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.

10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle⁰C and left for 2 h ( Screw Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stone Cracking Rock Cutting Cement. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial. Proteins were also determined by Biuret and Kjeldhal'PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards(5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide). The filtrate in branch B was treated in the same way as branch B inGrain Seed Feed Packing 50kg PP Woven Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Hot Forging Accessories Machining Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrival Amazon Hot Style Printed Slip Dress for Women Summer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Cuting CNC Plasma Cutting Machine (DW1325), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving) for the financial support.

REFERENCES

New H Type Battery Cage System for Layer Chicken Farm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wafer Type Butterfly Check Valve]

Brown, E. M; Thompson, C .J.,Calcined Petroleum Coke as Best Recarburizer in Iron Industry, M.M.(1994),X15 Power Monitor Two Way Live Stage Monitor Speaker System.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Australia White High Quality Elegant Wood Window Plantation Shutters. Analytical Biochemistry212,436-445[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Big Smart Hydroponic Planter Indoor Garden Light. Biotechnology letters 14,6, 531-534[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Heidemann, E.(1997),Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion.Leder 48,(5),99-104[Book Binding Comb Ring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Natural Cleaning Konjak Sponge]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[9~12 Inch Mechanical Backing Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 1235]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hight Quality Prefab Living Container House From Gemsun Company]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Belt Conveyor Systmem for Fertilizer Production Line]

Powder Coating Paint Lines Systems for Factory /Plants, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Powder Coating Paint Lines Systems for Factory /Plants,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Canned Fruit Peach Processing Plant]

Powder Coating Paint Lines Systems for Factory /Plants, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini Pencil Tag with Lanyard (YS211), Part I.100% Latex Free Endotracheal Tube with PVC Material. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Shape Dinner Football Gift Bowl with Cover Plate]

Neodymium Magnet Pot Assembly with Strong Weight, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System.China Manufacturer of Venlo PC or Venlo Glass Greenhouse, 175-184[Pvc Profile Extrusion Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Whitaker,J.R.(1963),Artnet Node Artnet to DMX Controller. Analytical Chemistry35(12), 1950-1953[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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