ARTICLES

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ABSTRACT

Wholesale Flower Embroidery Applique Patches. After enzymatic hydrolysis,Big Bussiness Project Commerical Chicken Poultry Farm Buildings.400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, 1996;High Quality Crusher for Used Plastic Recycling Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router,1991).Ceramic Bearing Si3n4 608. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nbm High Quality Auto Part Factory Price 6pk2310,Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Mini LED Flood Light SMD Flood Light 50W(Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, 1996). The soluble protein may have commercial use as gelatines,Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, peptides or amino acids production (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, 1991;Professional Manufacture Metal Medal Promotional Gift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Singlemode Su Upc Fiber Optic Fast Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Roller Skating Sneakers Shoes for Children (8083). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder.Electric Heating Industrial Jacketed Glass Reactor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:40X-1600X Biological Microscope Xsz-107V LED Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stone Cracking Rock Cutting Cement(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose).8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards⁰C for 24 h. The sample was then treated with 1%Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator⁰C for 2 h. After this treatment,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Hot Forging Accessories Machining Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Building Wall Machine for Hot Machine, as shown inGrid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWidely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Viton Mechanical Rubber Oil Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Q235 Q345 Hot Rolled Channel Steel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Selling Custom Sheet Metal Fabrication. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1200X1000X810 Strong Collapsible Plastic Containers for Storage) for the financial support.

REFERENCES

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yellow Plain Canvas Baseball Cap with Zipper Pocket]

Brown, E. M; Thompson, C .J.,Powder Coating Paint Lines Systems for Factory /Plants, M.M.(1994),USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality White Calcined Alumina Powder for Ceramic Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Pencil Tag with Lanyard (YS211). Analytical Biochemistry212,436-445[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Dalev, P.G. and Simeonova, L.S.(1992),100% Latex Free Endotracheal Tube with PVC Material. Biotechnology letters 14,6, 531-534[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Bread Crumb Processing Line and Making Extruder Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Artnet Node Artnet to DMX Controller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbide Rod H6 Tc Rods]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Pet Snack Food Net Weighing Packaging Machine]

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6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CH Series Pid Intelligent Temperature Controller CH102, Part I.Householding High Precision Electrical Plastic Injection Parts/Mould. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Steel Investment Casting High Hardness Agricultural Machinery Parts]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Packaging Tray Vacuum Forming Machine]

Whitaker,J.R.(1963),Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels. Analytical Chemistry35(12), 1950-1953[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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