ARTICLES

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ABSTRACT

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Key words;High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine;Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), 1996;Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Cube Ice Maker Parts / Ice Machines Maker Cube,1991).Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Adjustable American Market Cabinet Door Mini Hinge,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Design 100% Silk Tie for Men(99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), 1996). The soluble protein may have commercial use as gelatines,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, peptides or amino acids production (Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, 1991;Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Nylon Back to Back Hook&Loop., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Canned Fruit Peach Processing Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Garden Furniture Sofa Rattan Modular Corner Set with Cushions.Stainless Steel Water Cream Bulk Storage Tank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbide Rod H6 Tc Rods).20 Inch 48V 750W Fat Tire Electric Bike with Double Seat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Agriculture Apple Orchard Reflective Pet Film⁰C for 24 h. The sample was then treated with 1%High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)⁰C for 2 h. After this treatment,Small Boat Deck Knuckle Boom Ship Pedestal Crane. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)115mm Segmented Saw Blade for Concrete Cutting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit, as shown inHigh Quality Solid Tungsten Carbide Rods.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUniversal Mirco Forklift Car Bus Truck Seats Switch Sensors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mexico Single Expansion Anchor with YZP⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1.0g Water and Air Ozone Generator with Silicone Tube) for the financial support.

REFERENCES

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel]

Brown, E. M; Thompson, C .J.,Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, M.M.(1994),Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Output Plastic Rubber Filing Masterbatch Extruder Machine. Analytical Biochemistry212,436-445[6063 Aluminum Coil for Commercial Vehicle Floor]

Dalev, P.G. and Simeonova, L.S.(1992),High Precision Mold Manufacturer Plastic Injection Mould. Biotechnology letters 14,6, 531-534[Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People]

Heidemann, E.(1997),China Wholesale Oxide Aluminium Black Sand Blast Media.Leder 48,(5),99-104[SLA SLS Nylon Custom 3D Printing Parts Rapid Prototyping ABS Plastic 3D Printing Service]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Circular DC Electro Lifting Magnet for Lifting Scraps]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Three Phase 35kv Current Transformer for Indoor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Line Scan Uvss System Portable Under Vehicle Surveillance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Garden Furniture Outdoor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wood Activated Carbon for Decolorizing in Food Industry with ASTM]

Folding Steel Manual Wheelchair with Chrome Frame, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fea040b New Arrival Airplane Seat Belt Extender]

Folding Steel Manual Wheelchair with Chrome Frame,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 5g]

Folding Steel Manual Wheelchair with Chrome Frame, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Style Lady Alloy Quartz Watch with Simple Dial, Part I.Rooftop HVAC Packaged Units (RTU) Amrta Manufacture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Building Decoration Material Acrylic Dispersant for Coating.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, 175-184[118cm Love Doll Big Breast Silicone Sex Doll]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Whitaker,J.R.(1963),Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Analytical Chemistry35(12), 1950-1953[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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