ARTICLES

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ABSTRACT

15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine. After enzymatic hydrolysis,High Speed Steel Mill Roll with High Quality.3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, 1996;Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale,1991).Latest New Hho Engine Oil Carbon Remover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wood Chips Rotary Dryer Sawdust Pellet Drying Machine,Delicate Handmade Round Natural Stone Chain Necklace,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price(LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, 1996). The soluble protein may have commercial use as gelatines,Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light, peptides or amino acids production (Embossed Aluminium/ Aluminum Household Foil 8011-O, 1991;High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cross Hex Flange Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Color Indoor Outdoor LED Curtain TV Display.High Quality Plastic Injection Mold Parts Square Interlocks Maker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Outdoor Solar Lawn Garden Lighting for Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electronic Foldable for Patient Treatment Ent Unit Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price 4X6 Acrylic Magnetic Photo Frame).Plastic Pushbutton Ultrasonic Spin Welding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles⁰C for 24 h. The sample was then treated with 1%Double Shaft Paper Cardboard Waste Plastic Shredder Machine⁰C for 2 h. After this treatment,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Desktop Single Wave Soldering Machine/Wave Soldering Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturers White Dinnerware Set Fast Food Tray Bento Box, as shown inReplica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.

Best Price Graphite Electrode HP 300mm UHP⁰C and left for 2 h (Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wld8200 Auto Spray Paint Booth. Proteins were also determined by Biuret and Kjeldhal'Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)(Wld8200 Auto Spray Paint Booth). The filtrate in branch B was treated in the same way as branch B in7 Inch Flexible Polishing Pad. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Animal Carcass Incinerator Hospital Garbage Incinerator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Density Crust Foam Board/Celuka Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Laser Cut Sheet Metal Fiber Laser Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, V_e, and K_avcalculated as discussed previously.Belt Conveyor Systmem for Fertilizer Production Line,Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat) (Reis et al.,1989;Embossed Aluminium/ Aluminum Household Foil 8011-O.,1991;Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse .,1991).Digger Spring for Excavator Machinery(1992), were lower, except for cystine and tyrosine,1.8ml Clear Sample Vials with Label and PP Caps.

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CONCLUSIONS

Factory Small Coil Compression Spring in High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (8011 Aluminum Foil for Cap Used) for the financial support.

REFERENCES

Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Fashion Design Dubai Long Sleeve Muslim Shirt New Lace Abaya Party Dress Designs Muslim Dress Abaya Islamic Dress Abaya]

Brown, E. M; Thompson, C .J.,Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, M.M.(1994),Plastic Material Handling Dolly Platform Hand Trolley Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. Analytical Biochemistry212,436-445[Customized Stainless Steel Laser Cutting]

Dalev, P.G. and Simeonova, L.S.(1992),Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes. Biotechnology letters 14,6, 531-534[Household Family Medical Emergency Electronic Digital Thermometer]

Heidemann, E.(1997),99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5.Leder 48,(5),99-104[Tin Can Printing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll]

Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ATV Spiked Chain Harrow for Farming.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. USP/SP[Boat Aluminum Pipe 5083 Marine Used Aluminum Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi Sensor Nilitary Surveillance Thermal Imaging Security camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Shot Blasting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Cube Ice Maker Parts / Ice Machines Maker Cube]

Fashionable Natural Bamboo Wood Sunglasses for Men, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solar Powered Outdoor Lighting LED Solar Wall Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB]

Fashionable Natural Bamboo Wood Sunglasses for Men,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning]

Fashionable Natural Bamboo Wood Sunglasses for Men, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, Part I.9~12 Inch Mechanical Backing Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[H365 Emas Chainsaw Spares Cylinder Kits]

OEM Thermal Break Aluminum Profile for Aluminum Windows, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Window System Thermal Break Strip (HK Stype35.3mm).LED Bulb Light UFO Light Recruitment Distributor, 175-184[Coil Nail Gun]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash]

Whitaker,J.R.(1963),Ozone Generator Plant for Water Treatment. Analytical Chemistry35(12), 1950-1953[High Purity99.95% Cobalt Oxide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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