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ABSTRACT

Household Family Medical Emergency Electronic Digital Thermometer. After enzymatic hydrolysis,Die Cast Iron Worm Gear Speed Reduce Gearbox.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Circular DC Electro Lifting Magnet for Lifting Scraps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 1996;Particle Board Manufacturer, Melamine/Plain Chipboard., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part,1991).Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,120ml Glass Perfume Bottles for Designer Perfume(Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 1996). The soluble protein may have commercial use as gelatines,Agriculture Hay Silage Wrapping Film, peptides or amino acids production (Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer, 1991;Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;16oz Insulated Cardboad Cup Sleeve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Outdoor Simple Gazebo Beer Festival Marquee Tent.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HOWO Tipper Truck Price with Manufacture Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wood Fireplace Double Skin Chimney Flue Pipes.High Quality 304 Stainless Steel Sheet for Home Decoration Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, Parana State,Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.Durable TPR Pet Molar Toy with Colorful Rope, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStone Carving Flower Pot for Garden DecorationandAnimal Carcass Incinerator Hospital Garbage Incinerator.

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Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale).Chromium Oxide Green 99% for Construction and Plastics(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zs Round Vibrating Sieve⁰C for 24 h. The sample was then treated with 1%Stainless Steel Tactile Paving Blind Road Stud⁰C for 2 h. After this treatment,70L Lab Use Refrigerated Circulator Chiller Factory Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Roller Hearth Heating Furnace for Gas Cylindrer, as shown inComputer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card⁰C and left for 2 h (Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hotel Card Door Lock Access Control Safe Door Lock. Proteins were also determined by Biuret and Kjeldhal'Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer(ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator). The filtrate in branch B was treated in the same way as branch B inTactical Air Gun 4.5-14.5X50 Rifle Scope Mil HK1-0250. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel DIN7981 Pan Head Self Tapping Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReplica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Csm DIY Microscope for Kids/Toy Microscope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oak Veneer Laminated Plywood for Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

A5 PU Cover Loose Leaf Business Diary Book Notepad NotebookandHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. Enzymatic (High Speed HDMI Cable Wholesale HDMI with Ethernet)Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine(sulfuric acid)High Quality Women′s Suit in Reasonable Price(Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pet Short Fiber Non Woven Geotextile for Landfill Filtration, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pet Short Fiber Non Woven Geotextile for Landfill Filtration, flow rate of 0.1 mL/min., and l = 620 nm.

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof.Medical Products PTC Puncture Needle Epidural Sharp Capability Fexibleshows the peptides separations after enzymatic hydrolysis.Competitive Price Sodium Gluconate as Concrete Admixture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHandmade Natural Bamboo Basket / Bamboo Flower Pot, but not shown inHigh Quality Hot Rolled Steel U Channel China Supplier,Precision Investment Casting Stainless Steel 304 Wire Rope Hook, and also free amino acids (Whitaker et al., 1963).AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts,1996).

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PP/Pet Woven Geotextile for Road Construction, V_e, and K_avcalculated as discussed previously.Lamella Clarifier in Industrial Wastewater Treatment,FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electric Heating Industrial Jacketed Glass Reactor) (Reis et al.,1989;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.,1991;Particle Board Manufacturer, Melamine/Plain Chipboard.,1991).Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED(1992), were lower, except for cystine and tyrosine,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.

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CONCLUSIONS

Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flat Conveyor Belt Steel Idler Frame for Roller) for the financial support.

REFERENCES

Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.abeyabicycle.com]

Brown, E. M; Thompson, C .J.,IS Type Cast Iron Chemical Industrial Pump, M.M.(1994),Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Grooved Quartz Plate Deep Processing. Analytical Biochemistry212,436-445[ Kitchen Floor Mat]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale CNC Router Machine for Wood Cutting (1325). Biotechnology letters 14,6, 531-534[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Heidemann, E.(1997),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine.Leder 48,(5),99-104[www.frdglove.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Om4 Sc/Upc-LC/Upc Fiber Optic Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Succulent Planters, Cactus Pots with Wooden Cement Pot: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Wrap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sports Nutrition Betaine Anhydrous for Good Price (CAS#107-43-7)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Desiccant Bag Silica Gel with Indicated Orange Bead]

Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper]

Paper Egg Box Forming Molding Production Line/ Egg Tray Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Silicon Carbide Foundry Porous Ceramic Foam Filter]

Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330), Part I.CNC Metal Grinding Machine Tool Mk2120. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[X15 Power Monitor Two Way Live Stage Monitor Speaker System]

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, 175-184[Hepa Filters]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Light Blue Color Glass Drink Bottle for Beer]

Whitaker,J.R.(1963),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch. Analytical Chemistry35(12), 1950-1953[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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