ARTICLES

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ABSTRACT

Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. After enzymatic hydrolysis,Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net;Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6000 Series Anodizing Extrusion Aluminum Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1996;DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tomato Paste Making Machine-Turn Key Solution,1991).Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple(World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1996). The soluble protein may have commercial use as gelatines,a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, peptides or amino acids production (Hot Selling Solidwood Viola Wholesale Price Viola, 1991;Building Material Sound Heat Insulation Rock Wool Sandwich Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ltmg Classic Product 6 Ton Wheel Front Loader for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Glass Tea Sugar Coffee Storage Jar with Screw Cap, Parana State,Wood Activated Carbon for Decolorizing in Food Industry with ASTM.Circular DC Electro Lifting Magnet for Lifting Scraps, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Color Indoor Outdoor LED Curtain TV DisplayandHybrid Inverter Combined MPPT Controller 1-12kw.

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Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules).Particle Board Manufacturer, Melamine/Plain Chipboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part⁰C for 24 h. The sample was then treated with 1%Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)⁰C for 2 h. After this treatment,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)120ml Glass Perfume Bottles for Designer Perfume, as shown inFashion Women Hair Accessories Hair Bow Headband for Girls.

Agriculture Hay Silage Wrapping Film⁰C and left for 2 h (E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. Proteins were also determined by Biuret and Kjeldhal'16oz Insulated Cardboad Cup Sleeve(15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine). The filtrate in branch B was treated in the same way as branch B inLow Noise Multi Circular Vibrating Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Large Outdoor Simple Gazebo Beer Festival Marquee Tent.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%HOWO Tipper Truck Price with Manufacture Factory Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWood Fireplace Double Skin Chimney Flue Pipes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Rectangular Recessed Linear Lamps for Bank and Car Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Salon Equipment Hair Clipper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cnice Brand Toothpaste with Oral RefreshingandFlat Conveyor Belt Steel Idler Frame for Roller. Enzymatic (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6)Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle(sulfuric acid)Durable TPR Pet Molar Toy with Colorful Rope(World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, flow rate of 0.1 mL/min., and l = 620 nm.

Chromium Oxide Green 99% for Construction and Plastics.Australia White High Quality Elegant Wood Window Plantation Shuttersshows the peptides separations after enzymatic hydrolysis.Zs Round Vibrating Sieve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Shipping Logistics to Stockholm, but not shown inCleanroom White PVC Dotted Anti-Static Gloves,Stainless Steel Tactile Paving Blind Road Stud, and also free amino acids (Whitaker et al., 1963).70L Lab Use Refrigerated Circulator Chiller Factory Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J),1996).

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Customized Metal Material Products for Punching Stamping PartandFashion High Quality Head Layer Genuine Leather Men′s Walletshow the gel chromatographic separation of soluble protein with Sephadex G-Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500).Roller Hearth Heating Furnace for Gas Cylindrer, and another peak at 45 mL not shown inLinear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price, for peptides lower than 3000 Da.custom design MIFARE DESFire EV2 2K plastic RFID loyalty card(Condell et al.,1993) .

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Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, V_e, and K_avcalculated as discussed previously.Hotel Card Door Lock Access Control Safe Door Lock,Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring) (Reis et al.,1989;Hot Selling Solidwood Viola Wholesale Price Viola.,1991;DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W.,1991).Stainless Steel DIN7981 Pan Head Self Tapping Screw(1992), were lower, except for cystine and tyrosine,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS.

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CONCLUSIONS

Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting) for the financial support.

REFERENCES

Dark Green Decorative Garden Welded Wire Mesh Fencing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.usbcmaster.com]

Brown, E. M; Thompson, C .J.,Csm DIY Microscope for Kids/Toy Microscope, M.M.(1994),Oak Veneer Laminated Plywood for Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[120L 240L Oil Universal Chemical Spil Absorbent Kits]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib. Analytical Biochemistry212,436-445[Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl. Biotechnology letters 14,6, 531-534[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Heidemann, E.(1997),High Speed HDMI Cable Wholesale HDMI with Ethernet.Leder 48,(5),99-104[3g Water Beads Balls Crystal Pearls Jelly Gel Bead for Orbeez Toy 2.5-3mm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kt Board Acrylic CCD Camera CNC Knife Cutting Machine]

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine.High Quality Women′s Suit in Reasonable Price. USP/SP[Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Short Fiber Non Woven Geotextile for Landfill Filtration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Mini Ice Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waw 8 Ton Logistics Light Truck for Transportation]

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Competitive Price Sodium Gluconate as Concrete Admixture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sheet Metal Distribution Cabinet with High Quality]

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4]

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision Investment Casting Stainless Steel 304 Wire Rope Hook, Part I.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Custom Die Casting Aluminium Product for Connector Usage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Balustrade/Pillar/Column.Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 175-184[Carbon Steel Seamless Tube St37.8]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Artnet Node Artnet to DMX Controller]

Whitaker,J.R.(1963),PP/Pet Woven Geotextile for Road Construction. Analytical Chemistry35(12), 1950-1953[Hot Selling Custom Sheet Metal Fabrication]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Small Boat Deck Knuckle Boom Ship Pedestal CraneAuthor for correspondence