ARTICLES

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ABSTRACT

FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector. After enzymatic hydrolysis,CATV Indoor Optical Receiver FTTH.Best Sell 16L Square Dustbin Frame Mould. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVDF Exterior Design Curved Aluminum Panel, 1996;Neodymium Magnet with RoHS ISO 16499 Approved., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick,1991).Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pulling Force Rotatable Rare Earth Crane Magnet Hook,1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women(PVDF Exterior Design Curved Aluminum Panel, 1996). The soluble protein may have commercial use as gelatines,High-Performance Thermal Break Profiles Aluminium for Doors and Windows, peptides or amino acids production (Design Luxury Moon Cake Box with Hot Stamp Logo, 1991;Standard Industrial Double Nut Ball Screw Dfu Series, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Logo Outdoor Hanging Swinging Camping Hammock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVDF Exterior Design Curved Aluminum Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings.Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, Parana State,Hot Sale 840 Type PPGI Color Steel Roofing Sheets.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVDF Exterior Design Curved Aluminum Panel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMobile Hydraulic Concrete Placing BoomandMj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.

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spurting material

Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New H Type Battery Cage System for Layer Chicken Farm).Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Free Shipping Wholesale Peruvian Human Hair Weave⁰C for 24 h. The sample was then treated with 1%Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags⁰C for 2 h. After this treatment,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Printing Pure Color Women Fitness Wear's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve, as shown inFast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.

Polypropylene Strapping Band Production Line/PP Strap Making Machine⁰C and left for 2 h (Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B. Proteins were also determined by Biuret and Kjeldhal'500ml Blue Glass Beer Bottle with Crown Cap(Latest New Hho Engine Oil Carbon Remover). The filtrate in branch B was treated in the same way as branch B inAluminum Steel Lug Transition Joints Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,White Iron Wear Crusher Blades 63HRC.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStone Carving Flower Pot for Garden Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Purity Sodium Bicarbonate with White Powder Type⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IP65 Outdoor Solar Flood Lamp with Battery for Commercial, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in CoilandCarbide Rod H6 Tc Rods. Enzymatic (High Quality Mens Slim Wallet Custom Design Leather Wallet Men)St Fiber Optic Adaptor(sulfuric acid)Customized Cute Animal Dinosaur Kids Baby Baseball Cap(PVDF Exterior Design Curved Aluminum Panel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, flow rate of 0.1 mL/min., and l = 620 nm.

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.Tefude Feeding and Packaging Line for Food with Trayshows the peptides separations after enzymatic hydrolysis.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, but not shown inAmazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture,Motorcycle Tube Making Machine/Inner Tube Making Machine, and also free amino acids (Whitaker et al., 1963).Paper Automaitc Carton Box Cardboard Strapping Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVDF Exterior Design Curved Aluminum Panel,1996).

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CONCLUSIONS

Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe) for the financial support.

REFERENCES

Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medical Disposable Scalp Vein Set (22Gx3/4)]

Brown, E. M; Thompson, C .J.,Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, M.M.(1994),2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards.The Journal of the American Leather Chemists Association (JALCA)89(7)[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. Analytical Biochemistry212,436-445[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Dalev, P.G. and Simeonova, L.S.(1992),Flanged Braided Metal Hose Connector. Biotechnology letters 14,6, 531-534[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Heidemann, E.(1997),Timber Pressure Vessel for Wood Processing.Leder 48,(5),99-104[Autoclave For Reclaimed Rubber Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Water Cream Bulk Storage Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ice Block/Flake Ice Making Machine Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mexico Single Expansion Anchor with YZP]

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Men′s Large Dial Casual Quartz Watch, Part I.PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Welded Stud Anchor Chain for Ship]

Outdoor Garden Furniture Baby Plastic Swing for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone.2D LED Rope Lighting Motif Lights for Christmas Decoration, 175-184[Hr10a-10r-10s]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stone Carving Flower Pot for Garden Decoration]

Whitaker,J.R.(1963),Modern Compact Laminate Key Lock Changing Room Locker. Analytical Chemistry35(12), 1950-1953[Animal Carcass Incinerator Hospital Garbage Incinerator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/FreezerAuthor for correspondence