ARTICLES

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ABSTRACT

Canned Fruit Peach Processing Plant. After enzymatic hydrolysis,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.Garden Furniture Sofa Rattan Modular Corner Set with Cushions. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Water Cream Bulk Storage Tank;Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996;1.0g Water and Air Ozone Generator with Silicone Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine,1991).16A/24A High Speed Wire Cable Braiding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot,5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,20 Inch 48V 750W Fat Tire Electric Bike with Double Seat(Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996). The soluble protein may have commercial use as gelatines,Agriculture Apple Orchard Reflective Pet Film, peptides or amino acids production (High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04), 1991;Small Boat Deck Knuckle Boom Ship Pedestal Crane, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;115mm Segmented Saw Blade for Concrete Cutting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Round Transparent Glass 750ml Liquor Glass Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D Creative Design Metal Medal Promotion Gift of Sport.Ad-L13 Black Perfume Plastics Vial Bottle 10ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mexico Single Expansion Anchor with YZP, Parana State,High Quality Nylon Back to Back Hook&Loop.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCold Drawn D2 H13 Alloy Steel Turning Rolland Metal Polishing Disc.

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SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20W Solar Street Light with Lens Motion Sensor 2 Years Warranty).Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Whole Absorbtion Flexo Printing Soltting Die Cutting Machine⁰C for 24 h. The sample was then treated with 1%Factory Price 4X6 Acrylic Magnetic Photo Frame⁰C for 2 h. After this treatment,200m Diesel Oil Hydraulic Water Well Drilling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Cmyk Printing Cake Gift Packing Box with Lid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Air Purification Activated Alumina, as shown inDental YAG Laser Welding Machine for Gold Silver Metal Jewelry.

Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)⁰C and left for 2 h (300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Resistan UV Grades PP Non Woven Fabric. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Scba Breathing Apparatus Equipment with Ce Certification. Proteins were also determined by Biuret and Kjeldhal'FRP Pultruded Pipe or GRP Profile(Competitive Price Sodium Gluconate as Concrete Admixture). The filtrate in branch B was treated in the same way as branch B inDIY Shade (WHP-455). The final soluble protein shown were used for peptides separations using gel permeation chromatography,6090 CO2 Sealed Glass Cooling Laser Cutting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sheet Metal Distribution Cabinet with High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Universal Mirco Forklift Car Bus Truck Seats Switch Sensors) for the financial support.

REFERENCES

Factory Supply Straight Ball Joint Bearing 02b04, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[55′′ Floor Outdoor Digital Signage]

Brown, E. M; Thompson, C .J.,Wrinkle Effect Crease UV Drying Machine UV Curing System, M.M.(1994),Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack.The Journal of the American Leather Chemists Association (JALCA)89(7)[Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. Analytical Biochemistry212,436-445[Wld8200 Auto Spray Paint Booth]

Dalev, P.G. and Simeonova, L.S.(1992),20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Biotechnology letters 14,6, 531-534[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

Heidemann, E.(1997),Tefude Feeding and Packaging Line for Food with Tray.Leder 48,(5),99-104[Ball Screw Support Ek10 Ek12 Ek15 Ek20]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[NPK 16X Hammer Spare Parts Racing Forged Piston]

Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. USP/SP[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4V330-10 Air Compressor Solenoid Industrial Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CMP No Nc Metal Switch Black 25mm Illuminated Push Button: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1 Ton Toyo Type Manual Chain Block]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Automatic Glue Dispensing System Machine Manufacturer for Electronic Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer]

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Price of a Retail Supermarket Push Cart for Sale]

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3bbl Brite Servicing Tank Beer Brewery Equipment Unitank]

Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Felt Disc Polishing Sanding Disc with Wool Felt Diam, Part I.13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[130kw 7 Degrees Scroll Type Industrial Water Chiller]

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth.240T goodeng water/oil/gas pipe drilling rig HDD machine, 175-184[LDPE Transparent Grip Seal Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat]

Whitaker,J.R.(1963),Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. Analytical Chemistry35(12), 1950-1953[Methyl Cyclohexane Methylcyclohexane]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sinotruk-HOWO 6X4 Tractor TruckAuthor for correspondence