ARTICLES

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ABSTRACT

Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. After enzymatic hydrolysis,Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbon Steel Stainless Steel Countersunk Head Hollow Bolt;Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, 1996;5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Clear PVC Roof Frame Tent in South Africa,1991).High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Bend Pipe for Industrial Use,Ybz-E16f20 Vane Steering Hydraulic Pumps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Snow White Pumpkin Seeds for Sale(Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, 1996). The soluble protein may have commercial use as gelatines,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, peptides or amino acids production (Automotive Acrylic Wholesale Masking Tape Factory Price, 1991;Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Watermrk Drop in Bathroom Basin Sink.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM ABS Industrial Plastic Sheet Injection Auto Part. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰15L Big Cooling Cylinder High Production Hard Ice Cream Machine.Flange Type Ball Float Water Tank Control Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pressure Braided Rubber Oil Hose(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Playground Plastic Slide with Swing and Basketry).High Quality Beech Wood Chiavari Chair at a Competitive(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gradient Print Overlay Push Button Membrane Keypad Switch⁰C for 24 h. The sample was then treated with 1%Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X⁰C for 2 h. After this treatment,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, as shown inBig Dimension UHP Graphite Electrodes for Steel Making.

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle⁰C and left for 2 h (Wholesale Promotion Snack Children School Lunch Bag Cooler Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Power Consumption Silicone Sex Doll Vending Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Toughened Glass Disc Suspension Insulator. Proteins were also determined by Biuret and Kjeldhal'Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller(Neodymium Magnet with RoHS ISO 16499 Approved). The filtrate in branch B was treated in the same way as branch B inEuropean Vertical Big Flower Ab with Embossed Fine Embossed Wallpaper Living Room Stereo Damascus Background Wallpaper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Round Airless Lotion Pump Bottles for Skin Care Products. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRound Shape Dinner Football Gift Bowl with Cover Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Alloy Rack for Conductor, Wire, Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Corrugated Carton Box Fully Automatic Folding Gluing MachineandOutdoor Accessories Fishing Hunting Masks. Enzymatic (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box)KERONG Security Safe Combination Smart Key Card Cabinet Door Lock(sulfuric acid)Cast Floating Ball Valve China Manufacturer (GAQ41F)(Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Table CNC Marble Engraver for Stone) for the financial support.

REFERENCES

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2016 Hot Sale Galvanized Chicken Layer Cages]

Brown, E. M; Thompson, C .J.,Fire Alarm Detector 24VDC Reflective Beam Detector, M.M.(1994),Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Mini Bag Spices Small Salt Packing Packaging Machine. Analytical Biochemistry212,436-445[Nbm High Quality Auto Part Factory Price 6pk2310]

Dalev, P.G. and Simeonova, L.S.(1992),No 55 Steel Wire Cross Type Razor Barbed Wire. Biotechnology letters 14,6, 531-534[Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations]

Heidemann, E.(1997),4V330-10 Air Compressor Solenoid Industrial Valve.Leder 48,(5),99-104[Economic Durable Artificial Grass for Your Leisure Backyard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder]

Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Various Standard Hot Sale Rotary Lab Single Round Punch.Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. USP/SP[LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biodegradable Food Packaging Take out Food Boxes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Light Blue Color Glass Drink Bottle for Beer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Furniture Hotel Dining Furniture Metal Hotel Table Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[608 Deep Groove Ball Bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Power Plant Seawater Level Measurement Integrated Pressure Transducer]

Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Price High Torque 12mm Low Speed Small Geared Motor]

Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Black White Stone 925 Silver Cross Pendants Necklace Jewelry]

Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, Part I.Waterproof PVDF Aluminum Curtain Wall Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machined Power Transmission Shaft Part]

Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Round Modern Furniture Leisure Sofa for Promotion.Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, 175-184[Grooved Bolts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes]

Whitaker,J.R.(1963),Sany Hg3333m Climbing Placing Boom. Analytical Chemistry35(12), 1950-1953[Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CREE Chip 24W 28W 30W LED DownlightAuthor for correspondence