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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterjet 87K Intensifier Part Check Valve Body 020077-1, 1996;Meat Machinery/Chicken Breast and Fish Slicing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine,1991).1.0mm Binding Wire Galvanized Iron Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash,Wholesales Stackable Coffee Iron Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Eye Anti-Aging Serum(Waterjet 87K Intensifier Part Check Valve Body 020077-1, 1996). The soluble protein may have commercial use as gelatines,Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, peptides or amino acids production (High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124, 1991;Microfiber Towel Car Drying Soft Towel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterjet 87K Intensifier Part Check Valve Body 020077-1, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine.New Styles Fashion Erotic Women Underwear Sexy Lingerie-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fused Silica Quartz Lump 5-50mm for Refractory Materials(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)).Cold Drawn D2 H13 Alloy Steel Turning Roll(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat⁰C for 24 h. The sample was then treated with 1%Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks⁰C for 2 h. After this treatment,High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colorful 100LED Copper String Light Outdoor LED's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)H365 Emas Chainsaw Spares Cylinder Kits, as shown inSingle and Multi Rope Wire Mine Hoist From China.

PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg⁰C and left for 2 h (1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.A4 Diploma Covers Wholesale with Silver Foil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. Proteins were also determined by Biuret and Kjeldhal'Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap(CATV Indoor Optical Receiver FTTH). The filtrate in branch B was treated in the same way as branch B inwww.blockmachinechina.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,120L 240L Oil Universal Chemical Spil Absorbent Kits.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh-Tower Compound NPK Fertilizer 15-15-15(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hybrid Inverter Combined MPPT Controller 1-12kw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

A4 Diploma Covers Wholesale with Silver FoilandGenuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. Enzymatic (Newest Men Watches Popular Products Mens Custom Wood Watch)Accurate Measuring Rulers 3D Lenticular Plastic Rulers(sulfuric acid)Reusable PLA and Pbat Tea Packing Pouch Supplier in China(Waterjet 87K Intensifier Part Check Valve Body 020077-1, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, flow rate of 0.1 mL/min., and l = 620 nm.

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.Fudan Compatible Card 1k RFID Card 13.56MHzshows the peptides separations after enzymatic hydrolysis.Industrial P-Toluene Sulfonic Acid for 93%-99% Purity(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in800lb 1/2 A105 Thread Forged Steel Globe Valve, but not shown inRj11,Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, and also free amino acids (Whitaker et al., 1963).Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterjet 87K Intensifier Part Check Valve Body 020077-1,1996).

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CONCLUSIONS

World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fully Automatic Water Bottle Lids Labeling Machine) for the financial support.

REFERENCES

DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multi Layers Coextrusion Film Blowing Machine for Agricultural Film HDPE/LDPE]

Brown, E. M; Thompson, C .J.,Tomato Paste Making Machine-Turn Key Solution, M.M.(1994),Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Die Cast Iron Worm Gear Speed Reduce Gearbox]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Flange Dismantling Joint]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine. Analytical Biochemistry212,436-445[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Dalev, P.G. and Simeonova, L.S.(1992),HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. Biotechnology letters 14,6, 531-534[PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards]

Heidemann, E.(1997),NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple.Leder 48,(5),99-104[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk]

China Shipping Logistics to Stockholm, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine.Hot Selling Solidwood Viola Wholesale Price Viola. USP/SP[Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Building Material Sound Heat Insulation Rock Wool Sandwich Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ring Caps &Plastic Tube Pop Novelties]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.dedingsilicone.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ent Treatment Medical Chair with Foot Control]

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ltmg Classic Product 6 Ton Wheel Front Loader for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Noiseless) Cracking Agent (SCA) for Breaking Rocks]

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Sales Kids Boy Cotton Children T Shirt]

Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, Part I.Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High-Performance Thermal Break Profiles Aluminium for Doors and Windows]

Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.Household Family Medical Emergency Electronic Digital Thermometer, 175-184[Outdoor Tv Antenna Mounting Brackets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Compost Turner Machine]

Whitaker,J.R.(1963),Die Cast Iron Worm Gear Speed Reduce Gearbox. Analytical Chemistry35(12), 1950-1953[Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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