ARTICLES

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ABSTRACT

Factory Direct Sale Patent up to Down Rubber Tile Curing Press. After enzymatic hydrolysis,Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China.Real Leather Wallet Mens Designer Leather Wallets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338);Full Port Small Automatic Electronic Plumbing Ball Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cleanroom White PVC Dotted Anti-Static Gloves, 1996;High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Marine Solas Adhesive Reflective Tape,1991).G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers,ASTM A240 304 316L No. 1 Stainless Steel Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12V 24V 220V Digital Temperature Controller(Cleanroom White PVC Dotted Anti-Static Gloves, 1996). The soluble protein may have commercial use as gelatines,Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, peptides or amino acids production (25kg Fertilizer Belt Packing Machine for Special Materials, 1991;Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rotor High Output Electric 120 Watt Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cleanroom White PVC Dotted Anti-Static Gloves, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rpl Straight Socket and Plugs Male Thread(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape, Parana State,Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cleanroom White PVC Dotted Anti-Static Gloves, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Produce Medical Machining Custom Metal MillingandAuto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.

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WPC Wood Plastic Board Equipment Extruder Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lifting Thread with Needle for Plastic Surgery).Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Neoprene Elbow Support⁰C for 24 h. The sample was then treated with 1%Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules⁰C for 2 h. After this treatment,CNC Produce Medical Machining Custom Metal Milling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multi Sensor Nilitary Surveillance Thermal Imaging Security camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Metal Stamping Welding Spare Parts for Farming, as shown inCompatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead.

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine⁰C and left for 2 h (BLE5.0 Ttl to Bluetooth Module with at Command). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,275ml Frosted Clear Glass Drinking Bottle for Juice. Proteins were also determined by Biuret and Kjeldhal'EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price(7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input). The filtrate in branch B was treated in the same way as branch B inCartridge Heaters MGO Powder Filling Machines. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bolts and Nuts Set Brass Fasteners Double Hex Bolt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Direct Sales Kids Boy Cotton Children T Shirt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Light Blue PVC Plastic Sheet and PVC Film, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0andMetric Female Cone Hydraulic Hose Fitting. Enzymatic (Hot Sale Dental Material Dental PMMA Block From China)Plastic Waste Pet Flakes Recycling Washing Machine(sulfuric acid)Deposited Stainless Steel Rivet Nut with Knurled(Cleanroom White PVC Dotted Anti-Static Gloves, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Stainless Steel Mechanical Filter Hosing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Stainless Steel Mechanical Filter Hosing, flow rate of 0.1 mL/min., and l = 620 nm.

Paper New Year Greeting Cards Wedding Invitations Cards.Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracketshows the peptides separations after enzymatic hydrolysis.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRJ45 Cat5e in-Line Coupler Modular Jack, but not shown in1.0g Water and Air Ozone Generator with Silicone Tube,BLE5.0 Ttl to Bluetooth Module with at Command, and also free amino acids (Whitaker et al., 1963).Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cleanroom White PVC Dotted Anti-Static Gloves,1996).

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Square Shape Glass Candle Holder for One Piece Tealight Candle, V_e, and K_avcalculated as discussed previously.1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box,Medical Autoclavable Surgical Thoracoscopy Trocar. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Brand New Forklift with Double Front pneumatic Tire) (Reis et al.,1989;25kg Fertilizer Belt Packing Machine for Special Materials.,1991;High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.,1991).DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(1992), were lower, except for cystine and tyrosine,Easy Foldable Kids Walking Chair Toys Baby Walker.

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CONCLUSIONS

Men V-Neck Longline T-Shirt with Color Block Ribbed Trims. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss Wafer Type Check Valve Dn32 Dn40 Dn50) for the financial support.

REFERENCES

Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Kids 100% Cotton Children Little Boys Girls T Shirt]

Brown, E. M; Thompson, C .J.,Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve, M.M.(1994),Outdoor Multifunctional Solar Power Flashlight (SYSG-517).The Journal of the American Leather Chemists Association (JALCA)89(7)[Commercial Inflatable Dry Slide for Kids]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Regular Size Cigarette Rolling Papers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product. Analytical Biochemistry212,436-445[ Melamine/Plain Chipboard]

Dalev, P.G. and Simeonova, L.S.(1992),Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass. Biotechnology letters 14,6, 531-534[Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert]

Heidemann, E.(1997),High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer.Leder 48,(5),99-104[Ca Fire New Product 1.3kg Fire Truck Stress Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Painted G80 Cargo Lashing Chain with Hooks]

Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),FC Bare Fiber Optic Adapter for Network.3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory. USP/SP[GRP/FRP Profile Structures/Fiberglass Pultrusion Product]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cattle Corpse Cremation Burning Stove: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ozone Generator Plant for Water Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.usbcmaster.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Scented Flavor Flower Herbal Tea]

L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 49]

L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, Part I.Professional Stainless Steel Biological Fermentor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h]

16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rj11, Rj12, RJ45 Network Cable Modular Plug Crimping Tool Lk-Nt005.Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing, The Assembly, The Packaging,and End of Life for The Mould and Mould Part, 175-184[Silicon Solar Pv Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller]

Whitaker,J.R.(1963),16 Spindle Lace High Speed Braiding Machine Textile Machinery. Analytical Chemistry35(12), 1950-1953[China Factory Light Soda Ash /Sodium Carbonate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge BatteryAuthor for correspondence