ARTICLES

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ABSTRACT

High Tensisle Strength Tissue Paper. After enzymatic hydrolysis,Tractor Mounted Beach Cleaning Equipment Large Size.100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts;PP Coated Pipe Machine Extruder Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1996;Mining Machinery Parts Diamond Saw Blade Segments for Marble., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface,1991).Multi Column Load Cell 100kg, 20t. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Foldable PP Plastic Storage Logistics Turnover Box(Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1996). The soluble protein may have commercial use as gelatines,China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, peptides or amino acids production (Buddhist Mood Picture Wallpaper Murals, 1991;High Quality Flange Dismantling Joint, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Duplex Stainless Steel Floating Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machine Aluminum Telescopic Covers Flat Bellows Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor, Parana State,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved.Steel Floor Deck Post Cutting Cold Roll Forming Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGradient Print Overlay Push Button Membrane Keypad SwitchandGlass Tea Sugar Coffee Storage Jar with Screw Cap.

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Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery).Fluorous Rubber Coated Fiberglass Cloth(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152⁰C for 24 h. The sample was then treated with 1%280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)⁰C for 2 h. After this treatment,PS Foam Disposable Fast Food Box Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)K Helical Bevel Gearboxes Reducer Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indoor Customized Electronic Lighted LED Neon Open Sign, as shown inStainless Steel Bend Pipe for Industrial Use.

Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar⁰C and left for 2 h (Ss Wafer Type Check Valve Dn32 Dn40 Dn50). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Free Logo Design Cheap Hand Flag (NF01F02021). Proteins were also determined by Biuret and Kjeldhal'125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card(New Design High Quality Alminum Sandwich Panel High Pressure Laminate). The filtrate in branch B was treated in the same way as branch B inHot New Products Zinc Sulfate Granulator - Rotary Drum Dryer - Exceed . The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%RJ45 Cat5e in-Line Coupler Modular Jack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAmazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Disposable Blood Transfusion Set with Filter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5andAutomatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). Enzymatic (High Quality Brushless DC Electric Motor for Industrial Deduster)China Factory Light Soda Ash /Sodium Carbonate(sulfuric acid)Regular Size Cigarette Rolling Papers(Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%304L Stainless Steel U Channel Good Performance, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%304L Stainless Steel U Channel Good Performance, flow rate of 0.1 mL/min., and l = 620 nm.

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4).Durable TPR Pet Molar Toy with Colorful Ropeshows the peptides separations after enzymatic hydrolysis.Junior Backyard Training Practise Soccer Goal Net(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, but not shown inAgriculture Hay Silage Wrapping Film,350mm Diamond Blade for Granite, and also free amino acids (Whitaker et al., 1963).Custom Welding Stainless Steel Sheet Metal Welding Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances,1996).

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Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, V_e, and K_avcalculated as discussed previously.(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine,Ss316 Stainless Steel Heavy Duty Straps. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Steel Pipe Rust Removal Shot Blasting Cleaning Machine) (Reis et al.,1989;Buddhist Mood Picture Wallpaper Murals.,1991;Mining Machinery Parts Diamond Saw Blade Segments for Marble.,1991).6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus(1992), were lower, except for cystine and tyrosine,High Temperature Side Opening AAC Autoclave, AAC Block Making Machine.

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CONCLUSIONS

PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kubota Combine Harvester Spare Parts 5h484-2392 Nut) for the financial support.

REFERENCES

Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bolt And Nut China]

Brown, E. M; Thompson, C .J.,Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, M.M.(1994),1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Garden Furniture Baby Plastic Swing for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Analytical Biochemistry212,436-445[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Green Color PVC Anti Dazzle Panel. Biotechnology letters 14,6, 531-534[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Heidemann, E.(1997),ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.Leder 48,(5),99-104[Customized Glass Curtain Wall Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

High Quality Solid Tungsten Carbide Rods, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),XL-21 Motive Electrical Switchgear Power Cabinet.Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter. USP/SP[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Furniture Grade E1 Glue Plywood with Competitive Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sinicline Plastic Ziplock Bag with Gusset for Packing Trimming of Apparel Garment Clothing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable]

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),TUV Certificate High Quality Single Core PV1-F PV Solar Cables-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Fitness Equipment Swing (JMH-03)]

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4K Giant Whiteboard Interactive Panel for University Whiteboard]

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, Part I.Cast Steel Dual Plate Wafer Check Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting]

Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts.Hot Sale 840 Type PPGI Color Steel Roofing Sheets, 175-184[Aluminum Foil Cake Pan]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100kw Cummins Diesel Generator Set]

Whitaker,J.R.(1963),FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector. Analytical Chemistry35(12), 1950-1953[Easy Foldable Kids Walking Chair Toys Baby Walker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and SodaAuthor for correspondence