ARTICLES

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ABSTRACT

Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator. After enzymatic hydrolysis,Carbon Steel Stainless Steel Countersunk Head Hollow Bolt.Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Clear PVC Roof Frame Tent in South Africa, 1996;High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Bend Pipe for Industrial Use,1991).Ybz-E16f20 Vane Steering Hydraulic Pumps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Snow White Pumpkin Seeds for Sale,ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automotive Acrylic Wholesale Masking Tape Factory Price(Clear PVC Roof Frame Tent in South Africa, 1996). The soluble protein may have commercial use as gelatines,Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, peptides or amino acids production (Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, 1991;Watermrk Drop in Bathroom Basin Sink, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM ABS Industrial Plastic Sheet Injection Auto Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.15L Big Cooling Cylinder High Production Hard Ice Cream Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flange Type Ball Float Water Tank Control Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Clear PVC Roof Frame Tent in South Africa, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring.Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Outdoor Plastic Chrome Auto Logo Signs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Pressure Braided Rubber Oil Hose, Parana State,Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.High Quality Beech Wood Chiavari Chair at a Competitive, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Clear PVC Roof Frame Tent in South Africa, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theT8 20W LED Grow Tube Supplementary Lighting Lamps for PlantandRotor High Output Electric 120 Watt Motor.

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Gradient Print Overlay Push Button Membrane Keypad Switch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings).Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)⁰C for 24 h. The sample was then treated with 1%Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform⁰C for 2 h. After this treatment,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Power Consumption Silicone Sex Doll Vending Machine, as shown inSteel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6).

Toughened Glass Disc Suspension Insulator⁰C and left for 2 h (Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. Proteins were also determined by Biuret and Kjeldhal'Customized Round Airless Lotion Pump Bottles for Skin Care Products(Salon Equipment Hair Clipper). The filtrate in branch B was treated in the same way as branch B inBattery Operated 3W COB LED Wall Small Switch Night Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Round Shape Dinner Football Gift Bowl with Cover Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Exporter of Wheat Flour Mill Plant (80t). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Alloy Rack for Conductor, Wire, Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Accessories Fishing Hunting Masks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2017 China Hot Sale Water Screen Movie FountainandKERONG Security Safe Combination Smart Key Card Cabinet Door Lock. Enzymatic (Cast Floating Ball Valve China Manufacturer (GAQ41F))PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda(sulfuric acid)Stainless Steel Main Pipe Tee Joint for Railway Piping System(Clear PVC Roof Frame Tent in South Africa, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Fire Alarm Detector 24VDC Reflective Beam Detector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Soundproof Construction Foam PVC/WPC Board) for the financial support.

REFERENCES

Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized High Pressure 6 Inch Layflat Hose / Pipe for Farm Irrigation]

Brown, E. M; Thompson, C .J.,Automatic Mini Bag Spices Small Salt Packing Packaging Machine, M.M.(1994),No 55 Steel Wire Cross Type Razor Barbed Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single and Multi Rope Wire Mine Hoist From China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4V330-10 Air Compressor Solenoid Industrial Valve. Analytical Biochemistry212,436-445[CATV Indoor Optical Receiver FTTH]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Various Standard Hot Sale Rotary Lab Single Round Punch. Biotechnology letters 14,6, 531-534[A4 Diploma Covers Wholesale with Silver Foil]

Heidemann, E.(1997),Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute.Leder 48,(5),99-104[Manufacturer 3G Fwp WCDMA UMTS Fixed Wireless Telephones]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fudan Compatible Card 1k RFID Card 13.56MHz]

China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Light Blue Color Glass Drink Bottle for Beer.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. USP/SP[800lb 1/2 A105 Thread Forged Steel Globe Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rj11]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ss316 Stainless Steel Heavy Duty Straps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disabled Lift Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof PVDF Aluminum Curtain Wall Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[USB LED Clock Fan USB-Powered Portable Fan with Clock]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, Part I.Round Modern Furniture Leisure Sofa for Promotion. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment]

Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sany Hg3333m Climbing Placing Boom.Plastic Packaging Tray Vacuum Forming Machine, 175-184[Tfw Approved Cast Iron Globe Valve Flanged]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System]

Whitaker,J.R.(1963),Permanent Suspended Magnetic Separator for Belt Conveyor. Analytical Chemistry35(12), 1950-1953[Longyi Crimping Types Cable Terminal Lugs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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