ARTICLES

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ABSTRACT

Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. After enzymatic hydrolysis,120ml Glass Perfume Bottles for Designer Perfume.Agriculture Hay Silage Wrapping Film. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer;Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.16oz Insulated Cardboad Cup Sleeve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1996;HOWO Tipper Truck Price with Manufacture Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wood Fireplace Double Skin Chimney Flue Pipes,1991).High Quality 304 Stainless Steel Sheet for Home Decoration Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant,LED Rectangular Recessed Linear Lamps for Bank and Car Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Salon Equipment Hair Clipper(Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1996). The soluble protein may have commercial use as gelatines,Flat Conveyor Belt Steel Idler Frame for Roller, peptides or amino acids production (LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, 1991;Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Durable TPR Pet Molar Toy with Colorful Rope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chromium Oxide Green 99% for Construction and Plastics. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Zs Round Vibrating Sieve.Stainless Steel Tactile Paving Blind Road Stud-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:70L Lab Use Refrigerated Circulator Chiller Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hotel Card Door Lock Access Control Safe Door Lock, Parana State,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air HoseandBrand New Forklift with Double Front pneumatic Tire.

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Stainless Steel DIN7981 Pan Head Self Tapping Screw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Mens Slim Wallet Custom Design Leather Wallet Men).19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax⁰C for 24 h. The sample was then treated with 1%Dark Green Decorative Garden Welded Wire Mesh Fencing⁰C for 2 h. After this treatment,Csm DIY Microscope for Kids/Toy Microscope. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oak Veneer Laminated Plywood for Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, as shown inCommercial Inflatable Dry Slide for Kids.

Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl⁰C and left for 2 h (Regular Size Cigarette Rolling Papers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed HDMI Cable Wholesale HDMI with Ethernet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine. Proteins were also determined by Biuret and Kjeldhal'High Quality Women′s Suit in Reasonable Price( Melamine/Plain Chipboard). The filtrate in branch B was treated in the same way as branch B inwww.tysonleather.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pet Short Fiber Non Woven Geotextile for Landfill Filtration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCompetitive Price Sodium Gluconate as Concrete Admixture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Die Casting Aluminium Product for Connector Usage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Balustrade/Pillar/Column, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide InsertandUniversal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. Enzymatic (PP/Pet Woven Geotextile for Road Construction)Lamella Clarifier in Industrial Wastewater Treatment(sulfuric acid)FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering(Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.Painted G80 Cargo Lashing Chain with Hooksshows the peptides separations after enzymatic hydrolysis.Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGRP/FRP Profile Structures/Fiberglass Pultrusion Product, but not shown inEngineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, and also free amino acids (Whitaker et al., 1963).IS Type Cast Iron Chemical Industrial Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Large Outdoor Simple Gazebo Beer Festival Marquee Tent,1996).

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Wholesale Scented Flavor Flower Herbal TeaandOEM Metal Stamping Stainless Steel Parts with CNC Machiningshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.Custom Grooved Quartz Plate Deep Processing, and another peak at 45 mL not shown in 49, for peptides lower than 3000 Da.Hot Sale CNC Router Machine for Wood Cutting (1325)(Condell et al.,1993) .

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Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, V_e, and K_avcalculated as discussed previously.50g Body Luxury King Crown Cosmetic Containers,Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller) (Reis et al.,1989;LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6.,1991;HOWO Tipper Truck Price with Manufacture Factory Price.,1991).Mini Succulent Planters, Cactus Pots with Wooden Cement Pot(1992), were lower, except for cystine and tyrosine,Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.

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CONCLUSIONS

4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (custom design MIFARE DESFire EV2 2K plastic RFID loyalty card) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,CNC Metal Grinding Machine Tool Mk2120, M.M.(1994),Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Csm DIY Microscope for Kids/Toy Microscope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. Analytical Biochemistry212,436-445[High Quality Electronic Circuit Board Supplier PCB Assembly]

Dalev, P.G. and Simeonova, L.S.(1992),Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. Biotechnology letters 14,6, 531-534[70L Lab Use Refrigerated Circulator Chiller Factory Price]

Heidemann, E.(1997),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.Leder 48,(5),99-104[2 USB Port QC 3.0 Socket AMP Mobile Phone USB Car Charger]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Duplex Stainless Steel Floating Ball Valve]

CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. USP/SP[Flexible Vj Rigid Shaft Coupling for Pipe Flange]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mashroom Head Nylon Anchor with Pozi Nail]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100ml Caviar Glass Jar Sealed Jam Jar/Glass Bottle for Food]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Muliti-Size Light Hot Stamping Foil for PS Frame]

Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price]

Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS]

Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), Part I.Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 6063]

High Quaity China Supplier Truck Trailer Auto Car Accessories, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light, 175-184[Stainless Steel Whisky Chilling Stones]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Baby Clothes Wear Fleece Baby Bunting Onesie Jacket]

Whitaker,J.R.(1963),Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. Analytical Chemistry35(12), 1950-1953[Cheap Rapid Prototype Leather Logo Stamping Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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