ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Indoor Load Break Switch Isolating Switch. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Biodegradable Disposible Straw Pulp Food Box Ppp-1007

ABSTRACT

T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card. After enzymatic hydrolysis,Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.NBR Latex Adhesive for Glove. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cheap Wooden House Zinc Plated Angle Bracket;4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, 1996;Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer,1991).500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12 Inch King Mattress Latex Foam Memory Foam Mattress,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, 1996). The soluble protein may have commercial use as gelatines,Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt, peptides or amino acids production (Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable, 1991;Longyi Crimping Types Cable Terminal Lugs, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rigid PVC Foam Board for FRP Marine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Furniture Gold PVD Plasma Coating Machine.Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Pipe Rust Removal Shot Blasting Cleaning Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Injection Molding Companies China for Tablet PC Case(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light).27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Solid Tungsten Carbide Rods⁰C for 24 h. The sample was then treated with 1%Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover⁰C for 2 h. After this treatment,Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Tensisle Strength Tissue Paper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tractor Mounted Beach Cleaning Equipment Large Size, as shown inNew Type Automatic Multihead Weigher All-in-One Packing Machine for Rice.

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap⁰C and left for 2 h (Fire Alarm Detector 24VDC Reflective Beam Detector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PP Coated Pipe Machine Extruder Machine. Proteins were also determined by Biuret and Kjeldhal'China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts(Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20). The filtrate in branch B was treated in the same way as branch B inFamiliar with Euro and Us Market High Visibility Reflective Clothes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mining Machinery Parts Diamond Saw Blade Segments for Marble. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foldable PP Plastic Storage Logistics Turnover Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant MachineandChina Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Enzymatic (Buddhist Mood Picture Wallpaper Murals)High Quality Flange Dismantling Joint(sulfuric acid)Duplex Stainless Steel Floating Ball Valve(Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, flow rate of 0.1 mL/min., and l = 620 nm.

Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine.Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse shows the peptides separations after enzymatic hydrolysis.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, but not shown inDesign Luxury Moon Cake Box with Hot Stamp Logo,Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, and also free amino acids (Whitaker et al., 1963).CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw,1996).

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Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, V_e, and K_avcalculated as discussed previously.HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor,Steel Floor Deck Post Cutting Cold Roll Forming Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Teal Blue Padded Bubble Mailer Envelopes) (Reis et al.,1989;Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable.,1991;Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag.,1991).Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)(1992), were lower, except for cystine and tyrosine,Fluorous Rubber Coated Fiberglass Cloth.

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CONCLUSIONS

Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Bread Crumb Processing Line and Making Extruder Machine) for the financial support.

REFERENCES

280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[80W All in One Outdoor Waterproof Solar Street Light with IP Camera]

Brown, E. M; Thompson, C .J.,PS Foam Disposable Fast Food Box Making Machine, M.M.(1994),K Helical Bevel Gearboxes Reducer Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Indoor Customized Electronic Lighted LED Neon Open Sign. Analytical Biochemistry212,436-445[Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor]

Dalev, P.G. and Simeonova, L.S.(1992),Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Biotechnology letters 14,6, 531-534[Custom Metal Stamping Welding Spare Parts for Farming]

Heidemann, E.(1997),Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button.Leder 48,(5),99-104[Beach Cruise Bike]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[200m Diesel Oil Hydraulic Water Well Drilling Machine]

Natural Cleaning Konjak Sponge, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Free Logo Design Cheap Hand Flag (NF01F02021).125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card. USP/SP[Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Creative Design Metal Medal Promotion Gift of Sport]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Meat Slicer Stainless]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man]

RJ45 Cat5e in-Line Coupler Modular Jack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale]

RJ45 Cat5e in-Line Coupler Modular Jack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

RJ45 Cat5e in-Line Coupler Modular Jack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, Part I.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment]

High Quality Disposable Blood Transfusion Set with Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle.Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), 175-184[m.handypowercord.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Bulb Light UFO Light Recruitment Distributor]

Whitaker,J.R.(1963),High Quality Brushless DC Electric Motor for Industrial Deduster. Analytical Chemistry35(12), 1950-1953[High Quality Women′s Suit in Reasonable Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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