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ABSTRACT

OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings. After enzymatic hydrolysis,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap.120L 240L Oil Universal Chemical Spil Absorbent Kits. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, 1996;Hybrid Inverter Combined MPPT Controller 1-12kw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery,1991).Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Newest Men Watches Popular Products Mens Custom Wood Watch,Accurate Measuring Rulers 3D Lenticular Plastic Rulers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reusable PLA and Pbat Tea Packing Pouch Supplier in China(8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, 1996). The soluble protein may have commercial use as gelatines,Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, peptides or amino acids production (Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, 1991;Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Purity99.95% Cobalt Oxide. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wood Activated Carbon for Decolorizing in Food Industry with ASTM.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Tomato Paste Making Machine-Turn Key Solution(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30).Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine⁰C for 24 h. The sample was then treated with 1%HPMC Similiar to Tylose Powder for Rapid-Set Adhesive⁰C for 2 h. After this treatment,NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Selling Solidwood Viola Wholesale Price Viola, as shown inWorld-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J).

Building Material Sound Heat Insulation Rock Wool Sandwich Panel⁰C and left for 2 h (Tomato Paste Making Machine-Turn Key Solution). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ltmg Classic Product 6 Ton Wheel Front Loader for Sale. Proteins were also determined by Biuret and Kjeldhal'Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror(Printing Pure Color Women Fitness Wear). The filtrate in branch B was treated in the same way as branch B inSingle Wall Shaker Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrecision Cold Runner Single Cavity Plastic Injection Mould Auto Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Die Cast Iron Worm Gear Speed Reduce Gearbox⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Couple Watches Steel Material Gift Watch OEM in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Diatomite Earth Beer FilterandGlass Tea Sugar Coffee Storage Jar with Screw Cap. Enzymatic (Circular DC Electro Lifting Magnet for Lifting Scraps)Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts(sulfuric acid)Particle Board Manufacturer, Melamine/Plain Chipboard(8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, flow rate of 0.1 mL/min., and l = 620 nm.

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machineshows the peptides separations after enzymatic hydrolysis.Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Scba Breathing Apparatus Equipment with Ce Certification, but not shown inEmpty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer,Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, and also free amino acids (Whitaker et al., 1963).120ml Glass Perfume Bottles for Designer Perfume. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush,1996).

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16oz Insulated Cardboad Cup Sleeve, V_e, and K_avcalculated as discussed previously.Large Outdoor Simple Gazebo Beer Festival Marquee Tent,HOWO Tipper Truck Price with Manufacture Factory Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Medical Ultrasound Gel) (Reis et al.,1989;Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold.,1991;Hybrid Inverter Combined MPPT Controller 1-12kw.,1991).Wood Fireplace Double Skin Chimney Flue Pipes(1992), were lower, except for cystine and tyrosine,High Quality 304 Stainless Steel Sheet for Home Decoration Material.

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CONCLUSIONS

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated) for the financial support.

REFERENCES

LED Rectangular Recessed Linear Lamps for Bank and Car Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100ml Blue Essential Oil Glass Bottle]

Brown, E. M; Thompson, C .J.,Salon Equipment Hair Clipper, M.M.(1994),Flat Conveyor Belt Steel Idler Frame for Roller.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVDF Exterior Design Curved Aluminum Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Succulent Planters]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Analytical Biochemistry212,436-445[Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle. Biotechnology letters 14,6, 531-534[Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs]

Heidemann, E.(1997),Durable TPR Pet Molar Toy with Colorful Rope.Leder 48,(5),99-104[FRP Pultrusion Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump]

H365 Emas Chainsaw Spares Cylinder Kits, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.Chromium Oxide Green 99% for Construction and Plastics. USP/SP[PP Coated Pipe Machine Extruder Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100 Liter Cooking Pots for Jam]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Zs Round Vibrating Sieve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Autoclavable Surgical Thoracoscopy Trocar]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[99% Purity 0.5mg/10mg/10vial PT 141 Polypeptide for Sexual Disorders]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Temperature Side Opening AAC Autoclave]

Stainless Steel Tactile Paving Blind Road Stud, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),70L Lab Use Refrigerated Circulator Chiller Factory Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

Stainless Steel Tactile Paving Blind Road Stud,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

Stainless Steel Tactile Paving Blind Road Stud, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), Part I.Roller Hearth Heating Furnace for Gas Cylindrer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016.Hotel Card Door Lock Access Control Safe Door Lock, 175-184[Clutch Bag Ladies Designer Leather Purse Women Wallet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Table CNC Marble Engraver for Stone]

Whitaker,J.R.(1963),Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. Analytical Chemistry35(12), 1950-1953[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ad-L13 Black Perfume Plastics Vial Bottle 10mlAuthor for correspondence