ARTICLES

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ABSTRACT

Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. After enzymatic hydrolysis,White Desiccant Bag Silica Gel with Indicated Orange Bead.High Quality Iron Wire Mesh Chain Link Mesh Security Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, 1996;360 Degree Maverick Angler Fishing Kayak with Rotating Seat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera,1991).Wholesale Flower Embroidery Applique Patches. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Big Bussiness Project Commerical Chicken Poultry Farm Buildings,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20(Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, 1996). The soluble protein may have commercial use as gelatines,New Electric Piston Pump Coating Machine PT6900, peptides or amino acids production (200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, 1991;Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Crusher for Used Plastic Recycling Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ceramic Bearing Si3n4 608. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nbm High Quality Auto Part Factory Price 6pk2310.Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Mini LED Flood Light SMD Flood Light 50W(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White⁰C and left for 2 h (Hot Sale Dental Material Dental PMMA Block From China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stone Cracking Rock Cutting Cement. Proteins were also determined by Biuret and Kjeldhal'8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(Precision Customized CNC Machined Mild Steel Flange for Automobile). The filtrate in branch B was treated in the same way as branch B inAutomatic Z-Wave Door Lock System Exporter Hot Sale Product. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Building Wall Machine for Hot Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Cuting CNC Plasma Cutting Machine (DW1325), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Good Quality Outdoor Music Wireless Cooler Speaker Box.Plastic Pushbutton Ultrasonic Spin Welding Machineshows the peptides separations after enzymatic hydrolysis.OEM Viton Mechanical Rubber Oil Seal(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements, but not shown inMotorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L,Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, and also free amino acids (Whitaker et al., 1963).Q235 Q345 Hot Rolled Channel Steel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera,1996).

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Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10), V_e, and K_avcalculated as discussed previously.Pure Water Cutting Head Assy for Waterjet Cutting Machine,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment) (Reis et al.,1989;200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic.,1991;360 Degree Maverick Angler Fishing Kayak with Rotating Seat.,1991).Latest Lace Fabric Design Logo Flag Embroidery Patch(1992), were lower, except for cystine and tyrosine,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken.

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CONCLUSIONS

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Manufacture Metal Medal Promotional Gift) for the financial support.

REFERENCES

Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiberglass Rod Reinforcing FGRP Pole]

Brown, E. M; Thompson, C .J.,New H Type Battery Cage System for Layer Chicken Farm, M.M.(1994),Calcined Petroleum Coke as Best Recarburizer in Iron Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 5g]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-X15 Power Monitor Two Way Live Stage Monitor Speaker System. Analytical Biochemistry212,436-445[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Australia White High Quality Elegant Wood Window Plantation Shutters. Biotechnology letters 14,6, 531-534[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

Heidemann, E.(1997),Big Smart Hydroponic Planter Indoor Garden Light.Leder 48,(5),99-104[Hydraulic Automatic Press Brake Metal Bending Machine High Speed]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling Custom Sheet Metal Fabrication: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed HDMI Cable Wholesale HDMI with Ethernet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[C Channel Steel Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ SLA Shoes Prototyping for Auto Accessory]

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Powder Coating Paint Lines Systems for Factory /Plants-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sludge Oil Separation Centrifuge]

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, Part I.Mini Pencil Tag with Lanyard (YS211). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Real Leather Wallet Mens Designer Leather Wallets]

Whitaker,J.R.(1963),China Manufacturer of Venlo PC or Venlo Glass Greenhouse. Analytical Chemistry35(12), 1950-1953[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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