ARTICLES

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ABSTRACT

Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs. After enzymatic hydrolysis,Fused Silica Quartz Lump 5-50mm for Refractory Materials.Cold Drawn D2 H13 Alloy Steel Turning Roll. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Colorful 100LED Copper String Light Outdoor LED, 1996;H365 Emas Chainsaw Spares Cylinder Kits., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg,1991).A4 Diploma Covers Wholesale with Silver Foil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,120L 240L Oil Universal Chemical Spil Absorbent Kits(Colorful 100LED Copper String Light Outdoor LED, 1996). The soluble protein may have commercial use as gelatines,Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse, peptides or amino acids production (High-Tower Compound NPK Fertilizer 15-15-15, 1991;20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hybrid Inverter Combined MPPT Controller 1-12kw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Colorful 100LED Copper String Light Outdoor LED, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts.Newest Men Watches Popular Products Mens Custom Wood Watch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Accurate Measuring Rulers 3D Lenticular Plastic Rulers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, Parana State,New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera.Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Colorful 100LED Copper String Light Outdoor LED, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCanned Fruit Peach Processing PlantandRound Shape Dinner Football Gift Bowl with Cover Plate.

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High Purity99.95% Cobalt Oxide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts).Wood Activated Carbon for Decolorizing in Food Industry with ASTM(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car⁰C for 24 h. The sample was then treated with 1%Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine⁰C for 2 h. After this treatment,8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, as shown inLongkou Fushi Automatic PS Foam Thermocol Container Forming Machine.

6000 Series Anodizing Extrusion Aluminum Tube⁰C and left for 2 h (Rpl Straight Socket and Plugs Male Thread). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. Proteins were also determined by Biuret and Kjeldhal'Tomato Paste Making Machine-Turn Key Solution(Deep Hole Cylinder Boring Machine for Sale). The filtrate in branch B was treated in the same way as branch B inHigh Quality Polished No. 1 Stainless Steel Pipe (201. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHPMC Similiar to Tylose Powder for Rapid-Set Adhesive(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling Solidwood Viola Wholesale Price Viola, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Building Material Sound Heat Insulation Rock Wool Sandwich Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil BottleandSintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. Enzymatic (Ltmg Classic Product 6 Ton Wheel Front Loader for Sale)Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror(sulfuric acid)Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar(Colorful 100LED Copper String Light Outdoor LED, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, flow rate of 0.1 mL/min., and l = 620 nm.

Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts.Flat Conveyor Belt Steel Idler Frame for Rollershows the peptides separations after enzymatic hydrolysis.Household Family Medical Emergency Electronic Digital Thermometer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, but not shown in500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet,Die Cast Iron Worm Gear Speed Reduce Gearbox, and also free amino acids (Whitaker et al., 1963).19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Colorful 100LED Copper String Light Outdoor LED,1996).

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Newest Men Watches Popular Products Mens Custom Wood WatchandDiamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Bladesshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Couple Watches Steel Material Gift Watch OEM in China.Glass Tea Sugar Coffee Storage Jar with Screw Cap, and another peak at 45 mL not shown inLife Size 170cm Silicone Sex Dolls Real Love Doll for Male, for peptides lower than 3000 Da.Circular DC Electro Lifting Magnet for Lifting Scraps(Condell et al.,1993) .

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Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, V_e, and K_avcalculated as discussed previously.Particle Board Manufacturer, Melamine/Plain Chipboard,CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Accurate Measuring Rulers 3D Lenticular Plastic Rulers) (Reis et al.,1989;High-Tower Compound NPK Fertilizer 15-15-15.,1991;H365 Emas Chainsaw Spares Cylinder Kits.,1991).Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(1992), were lower, except for cystine and tyrosine,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert.

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CONCLUSIONS

Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold) for the financial support.

REFERENCES

120ml Glass Perfume Bottles for Designer Perfume, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Combined Bathroom Mirror]

Brown, E. M; Thompson, C .J.,Agriculture Hay Silage Wrapping Film, M.M.(1994),Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane. Analytical Biochemistry212,436-445[Flanged Braided Metal Hose Connector]

Dalev, P.G. and Simeonova, L.S.(1992),16oz Insulated Cardboad Cup Sleeve. Biotechnology letters 14,6, 531-534[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Heidemann, E.(1997),Large Outdoor Simple Gazebo Beer Festival Marquee Tent.Leder 48,(5),99-104[Factory Cheap Price Ceramic Bearing 627 RS ABEC 3 Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Universal Wholesale Hot Sale Custom Blank Keys]

Fully Automatic Water Bottle Lids Labeling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),HOWO Tipper Truck Price with Manufacture Factory Price.Wood Fireplace Double Skin Chimney Flue Pipes. USP/SP[Steel Setractable Rail Protection Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 304 Stainless Steel Sheet for Home Decoration Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Posture Corrector Back Support]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Main Pipe Tee Joint for Railway Piping System]

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Rectangular Recessed Linear Lamps for Bank and Car Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material]

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[600X600 mm Ugr 19 LED Panel Lights]

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Salon Equipment Hair Clipper, Part I.Flat Conveyor Belt Steel Idler Frame for Roller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle.Durable TPR Pet Molar Toy with Colorful Rope, 175-184[Wholesale Matte Red College Graduation Gown]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

Whitaker,J.R.(1963),Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. Analytical Chemistry35(12), 1950-1953[Cheap Wooden House Zinc Plated Angle Bracket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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