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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products. After enzymatic hydrolysis,Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women.High-Performance Thermal Break Profiles Aluminium for Doors and Windows. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Design Luxury Moon Cake Box with Hot Stamp Logo;Standard Industrial Double Nut Ball Screw Dfu Series; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, 1996;Customized Logo Outdoor Hanging Swinging Camping Hammock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings,1991).Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h,Metal Detector for Gold, Silver, etc Underground Metel Detector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hing Quality Rubber O Ring in Motor High Pressure(Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, 1996). The soluble protein may have commercial use as gelatines,Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, peptides or amino acids production (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1991;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Free Shipping Wholesale Peruvian Human Hair Weave.Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, Parana State,High Pulling Force Rotatable Rare Earth Crane Magnet Hook.500ml Blue Glass Beer Bottle with Crown Cap, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)andHigh Quality Rubber Pressed Mixing Machine with Ce Certification.

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White Iron Wear Crusher Blades 63HRC(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass).Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stone Carving Flower Pot for Garden Decoration⁰C for 24 h. The sample was then treated with 1%2018 New Fashion Men Canvas Messenger Bag⁰C for 2 h. After this treatment,High Purity Sodium Bicarbonate with White Powder Type. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)IP65 Outdoor Solar Flood Lamp with Battery for Commercial, as shown inISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors.

Carbide Rod H6 Tc Rods⁰C and left for 2 h (100% Latex Free Endotracheal Tube with PVC Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Mens Slim Wallet Custom Design Leather Wallet Men. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,St Fiber Optic Adaptor. Proteins were also determined by Biuret and Kjeldhal'Customized Cute Animal Dinosaur Kids Baby Baseball Cap(Solar Powered Outdoor Lighting LED Solar Wall Light). The filtrate in branch B was treated in the same way as branch B inCheap Excavators For Sale Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSinotruk-HOWO 6X4 Tractor Truck, Prime Mover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Paper Automaitc Carton Box Cardboard Strapping Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Motor Company Produce BLDC Fan Motor for Barn Vent Fan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Latest Lace Fabric Design Logo Flag Embroidery Patchand1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. Enzymatic (Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet)New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10(sulfuric acid)Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Polypropylene Strapping Band Production Line/PP Strap Making Machine) for the financial support.

REFERENCES

Fashion Men′s Large Dial Casual Quartz Watch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pu Foam Crown Moulding]

Brown, E. M; Thompson, C .J.,PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, M.M.(1994),Outdoor Garden Furniture Baby Plastic Swing for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lifting Thread with Needle for Plastic Surgery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. Analytical Biochemistry212,436-445[BLE5.0 Ttl to Bluetooth Module with at Command]

Dalev, P.G. and Simeonova, L.S.(1992),2D LED Rope Lighting Motif Lights for Christmas Decoration. Biotechnology letters 14,6, 531-534[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Heidemann, E.(1997),Modern Compact Laminate Key Lock Changing Room Locker.Leder 48,(5),99-104[Dw45 Acb Universal Circuit Breaker with Ce]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor Full Color LED Video Wall.180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). USP/SP[Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[RJ45 Cat5e in-Line Coupler Modular Jack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1.0g Water and Air Ozone Generator with Silicone Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pearl Pigment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Wholesale Oxide Aluminium Black Sand Blast Media]

Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quick Connect Camlock and Grooved Couplings Quick Fitting Type D-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Router for Engraving Cutting Carving Drilling Milling]

Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality White Calcined Alumina Powder for Ceramic Industry, Part I.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

100kw Cummins Diesel Generator Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.Luxury Hotel Furniture Antique Design Marble Side Table, 175-184[100W 150W Clothing Label CNC 1410 Laser Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

Whitaker,J.R.(1963),Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Analytical Chemistry35(12), 1950-1953[Brand New Forklift with Double Front pneumatic Tire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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