ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Pressure Braided Rubber Oil Hose

ABSTRACT

High Quality Beech Wood Chiavari Chair at a Competitive. After enzymatic hydrolysis,Gradient Print Overlay Push Button Membrane Keypad Switch.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004);Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, 1996;Low Power Consumption Silicone Sex Doll Vending Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Toughened Glass Disc Suspension Insulator,1991).Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,Customized Round Airless Lotion Pump Bottles for Skin Care Products,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Round Shape Dinner Football Gift Bowl with Cover Plate(AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, 1996). The soluble protein may have commercial use as gelatines,Professional Exporter of Wheat Flour Mill Plant (80t), peptides or amino acids production (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, 1991;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Alloy Rack for Conductor, Wire, Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Accessories Fishing Hunting Masks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰KERONG Security Safe Combination Smart Key Card Cabinet Door Lock.Cast Floating Ball Valve China Manufacturer (GAQ41F)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Full Automatic Paper Lunch Food Box Carton Forming Machine, Parana State,High Pressure Braided Rubber Oil Hose.Single Core Solid Copper Conductor Rg Coaxial Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAnaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent EndoscopeandFlying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.

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Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn).Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Baby Clothes Wear Fleece Baby Bunting Onesie Jacket⁰C for 24 h. The sample was then treated with 1%Life Size 170cm Silicone Sex Dolls Real Love Doll for Male⁰C for 2 h. After this treatment,Reflective Sheeting Material for Licence Plate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Grade Nitrous Oxide for Hot Sales's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, as shown inHigh Quality Plastic Injection Mold Parts Square Interlocks Maker.

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap⁰C and left for 2 h (High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fire Alarm Detector 24VDC Reflective Beam Detector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle. Proteins were also determined by Biuret and Kjeldhal'Automatic Mini Bag Spices Small Salt Packing Packaging Machine(Seasoned Cast Iron South Africa Three Legged Potjie Pot). The filtrate in branch B was treated in the same way as branch B inWall Mount Switch Control Cabinet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,No 55 Steel Wire Cross Type Razor Barbed Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4V330-10 Air Compressor Solenoid Industrial Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Various Standard Hot Sale Rotary Lab Single Round Punch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Light Blue Color Glass Drink Bottle for Beer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sany Hg3333m Climbing Placing Boom.High Quality Cheap Price Disposable IV Infusion Set with Precise Filtershows the peptides separations after enzymatic hydrolysis.Plastic Packaging Tray Vacuum Forming Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNatural Cleaning Konjak Sponge, but not shown inQ195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire,Permanent Suspended Magnetic Separator for Belt Conveyor, and also free amino acids (Whitaker et al., 1963).Double Sides Clear White Frosted Acrylic Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle,1996).

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1235andBelt Conveyor Systmem for Fertilizer Production Lineshow the gel chromatographic separation of soluble protein with Sephadex G-5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, and another peak at 45 mL not shown inChina Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, for peptides lower than 3000 Da.CNC Machined Power Transmission Shaft Part(Condell et al.,1993) .

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CONCLUSIONS

Factory Direct Sale Patent up to Down Rubber Tile Curing Press. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Promotion Snack Children School Lunch Bag Cooler Bag) for the financial support.

REFERENCES

Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory Welded Wire Mesh Fence Price]

Brown, E. M; Thompson, C .J.,Real Leather Wallet Mens Designer Leather Wallets, M.M.(1994),Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).The Journal of the American Leather Chemists Association (JALCA)89(7)[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rpl Straight Socket and Plugs Male Thread]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Port Small Automatic Electronic Plumbing Ball Valve. Analytical Biochemistry212,436-445[Deep Hole Cylinder Boring Machine for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,. Biotechnology letters 14,6, 531-534[Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle]

Heidemann, E.(1997),Cleanroom White PVC Dotted Anti-Static Gloves.Leder 48,(5),99-104[Jacquard Football Fans Acrylic Sports Warmer Scarf]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flat Conveyor Belt Steel Idler Frame for Roller]

China Soundproof Construction Foam PVC/WPC Board, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.Marine Solas Adhesive Reflective Tape. USP/SP[Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aws E7018 Low Carbon Steel Welding Electrode]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Small Lightweight Fashion Travel Satchel Sling Cross Body Shoulder Bag for Men]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Newest Men Watches Popular Products Mens Custom Wood Watch]

Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM A240 304 316L No. 1 Stainless Steel Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V 24V 220V Digital Temperature Controller, Part I.Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Design 100% Silk Tie for Men]

25kg Fertilizer Belt Packing Machine for Special Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.Rotor High Output Electric 120 Watt Motor, 175-184[For Circuit Protection Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

Whitaker,J.R.(1963),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. Analytical Chemistry35(12), 1950-1953[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2PC Flanged&ThreadedAuthor for correspondence