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ABSTRACT

40X-1600X Biological Microscope Xsz-107V LED Light. After enzymatic hydrolysis,6063 Aluminum Coil for Commercial Vehicle Floor.Silicon Carbide Foundry Porous Ceramic Foam Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1996;Stone Cracking Rock Cutting Cement., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial,1991).PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Hot Forging Accessories Machining Parts(10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1996). The soluble protein may have commercial use as gelatines,Building Wall Machine for Hot Machine, peptides or amino acids production (New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, 1991;Metal Cuting CNC Plasma Cutting Machine (DW1325), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Expanded Metal Infill Panel Metal Mesh Custom Made.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DC Motor for Massagers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Good Quality Outdoor Music Wireless Cooler Speaker Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hsac Shac Linear Guideway, Parana State,Electric Heating Industrial Jacketed Glass Reactor.Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBeautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam ToothpasteandLight Blue PVC Plastic Sheet and PVC Film.

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Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer).Pure Water Cutting Head Assy for Waterjet Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat⁰C for 24 h. The sample was then treated with 1%Latest Lace Fabric Design Logo Flag Embroidery Patch⁰C for 2 h. After this treatment,China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, as shown inPan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener.

New H Type Battery Cage System for Layer Chicken Farm⁰C and left for 2 h (En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Calcined Petroleum Coke as Best Recarburizer in Iron Industry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,X15 Power Monitor Two Way Live Stage Monitor Speaker System. Proteins were also determined by Biuret and Kjeldhal'Australia White High Quality Elegant Wood Window Plantation Shutters(Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable). The filtrate in branch B was treated in the same way as branch B inChina Temperature Controller 4-20ma Output. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Big Smart Hydroponic Planter Indoor Garden Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQ195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Powder Coating Paint Lines Systems for Factory /Plants, pH 3.25., mobile phase B: buffer solution of 0.01 mol/USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ATV Spiked Chain Harrow for FarmingandMini Pencil Tag with Lanyard (YS211). Enzymatic (100% Latex Free Endotracheal Tube with PVC Material)Neodymium Magnet Pot Assembly with Strong Weight(sulfuric acid)Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System(10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Manufacturer of Venlo PC or Venlo Glass Greenhouse, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Manufacturer of Venlo PC or Venlo Glass Greenhouse, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Q235 Q345 Hot Rolled Channel Steel) for the financial support.

REFERENCES

DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Prime Steel Angle]

Brown, E. M; Thompson, C .J.,Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), M.M.(1994),SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity.The Journal of the American Leather Chemists Association (JALCA)89(7)[300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Rubber Pressed Mixing Machine with Ce Certification. Analytical Biochemistry212,436-445[Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear]

Dalev, P.G. and Simeonova, L.S.(1992),Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. Biotechnology letters 14,6, 531-534[Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller]

Heidemann, E.(1997),4K Giant Whiteboard Interactive Panel for University Whiteboard.Leder 48,(5),99-104[Din Standard Spring Washer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Style Lady Alloy Quartz Watch with Simple Dial]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Waterproof Spc PVC Plank Rigid Core Click Lock Vinyl Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm]

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Practical Edible Silicone Kitchenware (YB-ty-29)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[16 Spindle Lace High Speed Braiding Machine Textile Machinery]

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)]

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4 Inch Quarter Round PCD Diamond Grinding Cup Wheel, Part I.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CH Series Pid Intelligent Temperature Controller CH102]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[16oz Insulated Cardboad Cup Sleeve]

Whitaker,J.R.(1963),Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump. Analytical Chemistry35(12), 1950-1953[Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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