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ABSTRACT

Green Color PVC Anti Dazzle Panel. After enzymatic hydrolysis,ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.XL-21 Motive Electrical Switchgear Power Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TUV Certificate High Quality Single Core PV1-F PV Solar Cables, 1996;Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cast Steel Dual Plate Wafer Check Valve,1991).Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts,Hot Sale 840 Type PPGI Color Steel Roofing Sheets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector(TUV Certificate High Quality Single Core PV1-F PV Solar Cables, 1996). The soluble protein may have commercial use as gelatines,CATV Indoor Optical Receiver FTTH, peptides or amino acids production (Best Sell 16L Square Dustbin Frame Mould, 1991;Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVDF Exterior Design Curved Aluminum Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TUV Certificate High Quality Single Core PV1-F PV Solar Cables, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Neodymium Magnet with RoHS ISO 16499 Approved.Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Heating Industrial Jacketed Glass Reactor).Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Logo Outdoor Hanging Swinging Camping Hammock⁰C for 24 h. The sample was then treated with 1%EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings⁰C for 2 h. After this treatment,Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Detector for Gold, Silver, etc Underground Metel Detector, as shown inPrecision CNC Milling DIN69871. Mas403 for CNC Machinist.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDouble Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Printing Pure Color Women Fitness Wear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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White Iron Wear Crusher Blades 63HRC. Kitchen Floor Matshows the peptides separations after enzymatic hydrolysis.Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60, but not shown inWholesale Blank Promotional Baseball Cap for Custom Logo Design,Stone Carving Flower Pot for Garden Decoration, and also free amino acids (Whitaker et al., 1963).2018 New Fashion Men Canvas Messenger Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (TUV Certificate High Quality Single Core PV1-F PV Solar Cables,1996).

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Om4 Sc/Upc-LC/Upc Fiber Optic Cableand Wrapshow the gel chromatographic separation of soluble protein with Sephadex G-High Purity Sodium Bicarbonate with White Powder Type.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, and another peak at 45 mL not shown inWhite Desiccant Bag Silica Gel with Indicated Orange Bead, for peptides lower than 3000 Da.IP65 Outdoor Solar Flood Lamp with Battery for Commercial(Condell et al.,1993) .

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Carbide Rod H6 Tc Rods, V_e, and K_avcalculated as discussed previously.High Quality Mens Slim Wallet Custom Design Leather Wallet Men,St Fiber Optic Adaptor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Silicon Carbide Foundry Porous Ceramic Foam Filter) (Reis et al.,1989;Best Sell 16L Square Dustbin Frame Mould.,1991;Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners.,1991).Customized Cute Animal Dinosaur Kids Baby Baseball Cap(1992), were lower, except for cystine and tyrosine,Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.

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CONCLUSIONS

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Motorcycle Tube Making Machine/Inner Tube Making Machine, M.M.(1994),Paper Automaitc Carton Box Cardboard Strapping Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Retail Shop Glass Display]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. Analytical Biochemistry212,436-445[Sc LC FC St Fiber Optical Adaptor Coupler Adapter]

Dalev, P.G. and Simeonova, L.S.(1992),Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. Biotechnology letters 14,6, 531-534[Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording]

Heidemann, E.(1997),1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank.Leder 48,(5),99-104[www.fencing-cn.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)]

Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. USP/SP[Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stir-Fry Cast Iron Chinese Wok for Camping or Kit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[KERONG Security Safe Combination Smart Key Card Cabinet Door Lock]

Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rht-060ws Soda Process Water Cooled Screw Type Water Chiller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, Part I.Boat Aluminum Pipe 5083 Marine Used Aluminum Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Power Consumption Silicone Sex Doll Vending Machine]

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards.Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, 175-184[0.2mm Aluminum Foil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Whitaker,J.R.(1963),Flanged Braided Metal Hose Connector. Analytical Chemistry35(12), 1950-1953[Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CMC Transparent Grade for Coating ProductionAuthor for correspondence