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ABSTRACT

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle. After enzymatic hydrolysis,Clear PVC Roof Frame Tent in South Africa.High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Bend Pipe for Industrial Use;Ybz-E16f20 Vane Steering Hydraulic Pumps; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality Snow White Pumpkin Seeds for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 1996;Automotive Acrylic Wholesale Masking Tape Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price,1991).Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Watermrk Drop in Bathroom Basin Sink,OEM ABS Industrial Plastic Sheet Injection Auto Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,15L Big Cooling Cylinder High Production Hard Ice Cream Machine(ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 1996). The soluble protein may have commercial use as gelatines,Flange Type Ball Float Water Tank Control Valve, peptides or amino acids production (925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring, 1991;Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Outdoor Plastic Chrome Auto Logo Signs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Table CNC Marble Engraver for Stone.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Soundproof Construction Foam PVC/WPC Board.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Pressure Braided Rubber Oil Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, Parana State,Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter PlotterandAutomatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container.

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AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine).Low Power Consumption Silicone Sex Doll Vending Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Toughened Glass Disc Suspension Insulator⁰C for 24 h. The sample was then treated with 1%Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller⁰C for 2 h. After this treatment,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Round Airless Lotion Pump Bottles for Skin Care Products's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Round Shape Dinner Football Gift Bowl with Cover Plate, as shown inKorea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence.

Professional Exporter of Wheat Flour Mill Plant (80t)⁰C and left for 2 h (High Quality Beech Wood Chiavari Chair at a Competitive). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. Proteins were also determined by Biuret and Kjeldhal'Aluminum Alloy Rack for Conductor, Wire, Cable(Gradient Print Overlay Push Button Membrane Keypad Switch). The filtrate in branch B was treated in the same way as branch B in3mm Pine Plywood High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Accessories Fishing Hunting Masks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKERONG Security Safe Combination Smart Key Card Cabinet Door Lock(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Main Pipe Tee Joint for Railway Piping System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Glass Tea Sugar Coffee Storage Jar with Screw CapandWholesale Promotion Snack Children School Lunch Bag Cooler Bag. Enzymatic (Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b)Full Automatic Paper Lunch Food Box Carton Forming Machine(sulfuric acid)Single Core Solid Copper Conductor Rg Coaxial Cable(ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use, flow rate of 0.1 mL/min., and l = 620 nm.

Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.Teflon Seal/Seals Spring Energizedshows the peptides separations after enzymatic hydrolysis.Baby Clothes Wear Fleece Baby Bunting Onesie Jacket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, but not shown inStainless Steel Bend Pipe for Industrial Use,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, and also free amino acids (Whitaker et al., 1963).Reflective Sheeting Material for Licence Plate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors,1996).

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CONCLUSIONS

Professional Various Standard Hot Sale Rotary Lab Single Round Punch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X) for the financial support.

REFERENCES

Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[250ml Honoy Glass Bottle Glass Jar with Plastic Lids]

Brown, E. M; Thompson, C .J.,Light Blue Color Glass Drink Bottle for Beer, M.M.(1994),Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Machinery Parts Aeroplane Engine CNC Milling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Analytical Biochemistry212,436-445[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Biotechnology letters 14,6, 531-534[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

Heidemann, E.(1997),Waterproof PVDF Aluminum Curtain Wall Panel.Leder 48,(5),99-104[New Style Mini Emergency Light Lamp Battery Backup LED Emergency Light Elevator Emergency Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Garden Furniture Baby Plastic Swing for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Bottle Unscrambler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Sany Hg3333m Climbing Placing Boom, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Packaging Tray Vacuum Forming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Sany Hg3333m Climbing Placing Boom,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

Sany Hg3333m Climbing Placing Boom, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Permanent Suspended Magnetic Separator for Belt Conveyor, Part I.Double Sides Clear White Frosted Acrylic Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.CNC Machined Power Transmission Shaft Part, 175-184[Women Waterproof Raincoat Outdoor Hooded Rain Jacket]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine]

Whitaker,J.R.(1963),Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. Analytical Chemistry35(12), 1950-1953[Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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