ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CMC Transparent Grade for Coating Production. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine

ABSTRACT

Cold-Spray Road Line Marking Machine. After enzymatic hydrolysis,Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner.Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rock Tunnel Boring Machine for Natural Gas Pipeline, 1996;NPK+Te Fertilizer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel,1991).Customized Double Acting Hydraulic Cylinder for Excavator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion High Quality Head Layer Genuine Leather Men′s Wallet,Teal Blue Padded Bubble Mailer Envelopes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Ultrasound Gel(Rock Tunnel Boring Machine for Natural Gas Pipeline, 1996). The soluble protein may have commercial use as gelatines,Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, peptides or amino acids production (Wholesale Blank Promotional Baseball Cap for Custom Logo Design, 1991;Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Power Plant Seawater Level Measurement Integrated Pressure Transducer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Melamine Paper Manufacturer for Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rock Tunnel Boring Machine for Natural Gas Pipeline, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema.Tungsten Solid Carbide Straight 4/6 Flutes Reamer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Quality Heavy Duty Globe Control Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ent Treatment Medical Chair with Foot Control(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Spark Resistant Construction Axial Fan with Fixed Pitch Blade).Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Leaf Shape Pearl Jewelry Wedding Tiara⁰C for 24 h. The sample was then treated with 1%Starter Motor 0001367034 12V 3KW 9T for Mercedes⁰C for 2 h. After this treatment,New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)HPMC Hydroxy Propyl Methyl Cellulose Additives's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste, as shown inBest Sell 16L Square Dustbin Frame Mould.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Water Chiller Air Cooled for Cooling Beer Refrigerating. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Cheap Price Disposable IV Infusion Set with Precise Filter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Big Dimension UHP Graphite Electrodes for Steel Making, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Design Pet Product, Climbing Willow Cat Scratcher Tree, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Shipping Logistics to Stockholm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements) for the financial support.

REFERENCES

CNC Lathe Main Parts with Good Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[WING MIRROR GLASS]

Brown, E. M; Thompson, C .J.,Good Price High Torque 12mm Low Speed Small Geared Motor, M.M.(1994),Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man. Analytical Biochemistry212,436-445[Small Table CNC Marble Engraver for Stone]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting. Biotechnology letters 14,6, 531-534[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

Heidemann, E.(1997),Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated.Leder 48,(5),99-104[7" Lcd Screen Boe 070 Rev.1]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Free Assembly, Walking Machine, Home Fitness, Treadmill, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mashroom Head Nylon Anchor with Pozi Nail.Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool. USP/SP[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterjet 87K Intensifier Part Check Valve Body 020077-1: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zhejiang Manufacture Explosion Proof Electric Actuator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Meat Machinery/Chicken Breast and Fish Slicing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500ml Blue Glass Beer Bottle with Crown Cap]

Meat Machinery/Chicken Breast and Fish Slicing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

Meat Machinery/Chicken Breast and Fish Slicing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.0mm Binding Wire Galvanized Iron Wire, Part I.IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chromium Oxide Green 99% for Construction and Plastics]

Wholesales Stackable Coffee Iron Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Eye Anti-Aging Serum.Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder, 175-184[Mother Of Pearl Earrings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Men′s Large Dial Casual Quartz Watch]

Whitaker,J.R.(1963),High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. Analytical Chemistry35(12), 1950-1953[High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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