ARTICLES

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ABSTRACT

Nbm High Quality Auto Part Factory Price 6pk2310. After enzymatic hydrolysis,Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay.High Quality Mini LED Flood Light SMD Flood Light 50W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Manufacture Metal Medal Promotional Gift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1996;Singlemode Su Upc Fiber Optic Fast Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Roller Skating Sneakers Shoes for Children (8083),1991).Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Heating Industrial Jacketed Glass Reactor,40X-1600X Biological Microscope Xsz-107V LED Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6063 Aluminum Coil for Commercial Vehicle Floor(Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1996). The soluble protein may have commercial use as gelatines,Silicon Carbide Foundry Porous Ceramic Foam Filter, peptides or amino acids production (1200X1000X810 Strong Collapsible Plastic Containers for Storage, 1991;Steel Construction in Poultry House with Good Quality and Installation, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stone Cracking Rock Cutting Cement. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial.PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Metal Cuting CNC Plasma Cutting Machine (DW1325), Parana State,Ceramic Bearing Si3n4 608.Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUniversal Mirco Forklift Car Bus Truck Seats Switch SensorsandChina Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring.

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Expanded Metal Infill Panel Metal Mesh Custom Made(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Design Pet Product).DC Motor for Massagers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage⁰C for 24 h. The sample was then treated with 1%Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry⁰C for 2 h. After this treatment,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Viton Mechanical Rubber Oil Seal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, as shown inHigh Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17.

Q235 Q345 Hot Rolled Channel Steel⁰C and left for 2 h (120L 240L Oil Universal Chemical Spil Absorbent Kits). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Factory New Girls Nuts Crunch Cheap Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hsac Shac Linear Guideway. Proteins were also determined by Biuret and Kjeldhal'Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses(Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950). The filtrate in branch B was treated in the same way as branch B inLed Touch Screen Smart Board. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pure Water Cutting Head Assy for Waterjet Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Building Wall Machine for Hot Machine) for the financial support.

REFERENCES

Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tbhq]

Brown, E. M; Thompson, C .J.,CH Series Pid Intelligent Temperature Controller CH102, M.M.(1994),Householding High Precision Electrical Plastic Injection Parts/Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Sides Clear White Frosted Acrylic Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Women Hair Accessories Hair Bow Headband for Girls. Analytical Biochemistry212,436-445[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Dalev, P.G. and Simeonova, L.S.(1992),Round Hole Carbon Steel Pipe Perforated for Filter. Biotechnology letters 14,6, 531-534[Rooftop HVAC Packaged Units (RTU) Amrta Manufacture]

Heidemann, E.(1997),Professional Manufacturer Stainless Steel Floor Grating.Leder 48,(5),99-104[4kw Fiber Laser Cutting Mchine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Roller Skating Sneakers Shoes for Children (8083)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paper Automaitc Carton Box Cardboard Strapping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12v Usb Circuit Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cast Steel Dual Plate Wafer Check Valve]

Brand New Forklift with Double Front pneumatic Tire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve]

Brand New Forklift with Double Front pneumatic Tire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[16A/24A High Speed Wire Cable Braiding Machine]

Brand New Forklift with Double Front pneumatic Tire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, Part I.Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Toughened Glass Disc Suspension Insulator]

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Rubber Pressed Mixing Machine with Ce Certification.Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600, 175-184[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quality Automatic High Speed Iron Wire Nail Concrete Steel Nail Forming Machine/Coil Nail Machine and Nail Thread Rolling Machine/Nail Twisting Machine]

Whitaker,J.R.(1963),4K Giant Whiteboard Interactive Panel for University Whiteboard. Analytical Chemistry35(12), 1950-1953[Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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