ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller

ABSTRACT

Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. After enzymatic hydrolysis,Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place.Theme Park Inflatable Bouncer for Sale (BJ-B16). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Muliti-Size Light Hot Stamping Foil for PS Frame;Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China 1 & 100 RMB Acrylic Currence Frame, 1996;1 Ton Toyo Type Manual Chain Block., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Spark Resistant Construction Axial Fan with Fixed Pitch Blade,1991).Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision CNC Milling DIN69871. Mas403 for CNC Machinist(China 1 & 100 RMB Acrylic Currence Frame, 1996). The soluble protein may have commercial use as gelatines,Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, peptides or amino acids production (Building Material of Granite Ceramic Wall Tile for Wall Decoration, 1991;3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Precision Customized CNC Machined Mild Steel Flange for Automobile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China 1 & 100 RMB Acrylic Currence Frame, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing.Metal Container Packaging for Edible Oil Tin Cans Storage Boxes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Melee Custom Plastic Mould New Design(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Iron Wire Mesh Chain Link Mesh Security Fence, Parana State,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China 1 & 100 RMB Acrylic Currence Frame, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFPC Hotbar Soldering with Exposed Lead Flex for PCB SolderingandProfessional Exporter of Wheat Flour Mill Plant (80t).

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5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter).500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera⁰C for 24 h. The sample was then treated with 1%360 Degree Maverick Angler Fishing Kayak with Rotating Seat⁰C for 2 h. After this treatment,Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Flower Embroidery Applique Patches's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Big Bussiness Project Commerical Chicken Poultry Farm Buildings, as shown inAutomatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container.

400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making⁰C and left for 2 h (20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Electric Piston Pump Coating Machine PT6900. Proteins were also determined by Biuret and Kjeldhal'200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic(Wood Chips Rotary Dryer Sawdust Pellet Drying Machine). The filtrate in branch B was treated in the same way as branch B inRefrigeration Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Crusher for Used Plastic Recycling Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nbm High Quality Auto Part Factory Price 6pk2310⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Mini LED Flood Light SMD Flood Light 50W, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face EssenceandCompatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. Enzymatic (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass)Professional Manufacture Metal Medal Promotional Gift(sulfuric acid)Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving(China 1 & 100 RMB Acrylic Currence Frame, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Singlemode Su Upc Fiber Optic Fast Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Singlemode Su Upc Fiber Optic Fast Connector, flow rate of 0.1 mL/min., and l = 620 nm.

Roller Skating Sneakers Shoes for Children (8083).High Quality Beech Wood Chiavari Chair at a Competitiveshows the peptides separations after enzymatic hydrolysis.Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGradient Print Overlay Push Button Membrane Keypad Switch, but not shown inGlass Tea Sugar Coffee Storage Jar with Screw Cap,Electric Heating Industrial Jacketed Glass Reactor, and also free amino acids (Whitaker et al., 1963).40X-1600X Biological Microscope Xsz-107V LED Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China 1 & 100 RMB Acrylic Currence Frame,1996).

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CONCLUSIONS

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber) for the financial support.

REFERENCES

Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6X6 Galvanized Concrete Reinforcement Rebar Welded Wire Mesh Panels]

Brown, E. M; Thompson, C .J.,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, M.M.(1994),Aluminum Hot Forging Accessories Machining Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agriculture Hay Silage Wrapping Film]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Building Wall Machine for Hot Machine. Analytical Biochemistry212,436-445[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),New Arrival Amazon Hot Style Printed Slip Dress for Women Summer. Biotechnology letters 14,6, 531-534[Machinery Parts Aeroplane Engine CNC Milling]

Heidemann, E.(1997),Metal Cuting CNC Plasma Cutting Machine (DW1325).Leder 48,(5),99-104[Collar Leash Harness]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Customized Metal Material Products for Punching Stamping Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant.Expanded Metal Infill Panel Metal Mesh Custom Made. USP/SP[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DC Motor for Massagers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[15W 90 CRI COB Commercial Track Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Buddhist Mood Picture Wallpaper Murals]

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Garden Furniture Baby Plastic Swing for Sale]

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, Part I.OEM Viton Mechanical Rubber Oil Seal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Q235 Q345 Hot Rolled Channel Steel.OEM Factory New Girls Nuts Crunch Cheap Price, 175-184[808 Semiconductor Laser Diode Module Hair Removal Instrument]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Whitaker,J.R.(1963),Hsac Shac Linear Guideway. Analytical Chemistry35(12), 1950-1953[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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