ARTICLES

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ABSTRACT

Cast Steel Dual Plate Wafer Check Valve. After enzymatic hydrolysis,Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products.Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CATV Indoor Optical Receiver FTTH, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Sell 16L Square Dustbin Frame Mould, 1996;Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush,1991).Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVDF Exterior Design Curved Aluminum Panel,Neodymium Magnet with RoHS ISO 16499 Approved,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick(Best Sell 16L Square Dustbin Frame Mould, 1996). The soluble protein may have commercial use as gelatines,Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, peptides or amino acids production (High Pulling Force Rotatable Rare Earth Crane Magnet Hook, 1991;1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Performance Thermal Break Profiles Aluminium for Doors and Windows.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Design Luxury Moon Cake Box with Hot Stamp Logo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Sell 16L Square Dustbin Frame Mould, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Standard Industrial Double Nut Ball Screw Dfu Series.Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Muliti-Size Light Hot Stamping Foil for PS Frame).Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2⁰C for 24 h. The sample was then treated with 1%Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair⁰C for 2 h. After this treatment,Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Free Shipping Wholesale Peruvian Human Hair Weave, as shown inJd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price.

Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags⁰C and left for 2 h (Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Printing Pure Color Women Fitness Wear. Proteins were also determined by Biuret and Kjeldhal'Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve( 6063). The filtrate in branch B was treated in the same way as branch B inProfessional Badminton Net. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polypropylene Strapping Band Production Line/PP Strap Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.White Iron Wear Crusher Blades 63HRC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stone Carving Flower Pot for Garden Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning) for the financial support.

REFERENCES

Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bra Sets For Women Push Up]

Brown, E. M; Thompson, C .J.,Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, M.M.(1994),Rht-060ws Soda Process Water Cooled Screw Type Water Chiller.The Journal of the American Leather Chemists Association (JALCA)89(7)[Round Plastic Hanging Basket (KD7014)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[K Helical Bevel Gearboxes Reducer Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. Analytical Biochemistry212,436-445[Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers]

Dalev, P.G. and Simeonova, L.S.(1992),Boat Aluminum Pipe 5083 Marine Used Aluminum Tube. Biotechnology letters 14,6, 531-534[High Quality Nylon Back to Back Hook&Loop]

Heidemann, E.(1997),Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar.Leder 48,(5),99-104[Round Rope/Round Wire Printing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles]

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards.Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. USP/SP[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Neoprene Elbow Support]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flanged Braided Metal Hose Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 304 Stainless Steel Sheet for Home Decoration Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Huawei E5172 4g Wifi Router]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Melee Custom Plastic Mould New Design]

Timber Pressure Vessel for Wood Processing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1200mm Recessed Mounted LED Linear Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Stainless Steel Biological Fermentor]

Timber Pressure Vessel for Wood Processing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Femo Ferro Molybdenum Powder Lump 55/60]

Timber Pressure Vessel for Wood Processing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:The Big Diameter Carbide Tipped Hole Saw, Part I.Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.Fashion Men′s Large Dial Casual Quartz Watch, 175-184[UHP Dia. 350mm (14inch) 1800mm (72inch) 3tpi Graphite Electrode]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automotive Rubber Parts for Any Rubber Material]

Whitaker,J.R.(1963),PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence. Analytical Chemistry35(12), 1950-1953[3-Pin Australia Power Cord Plug with SAA]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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