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ABSTRACT

Practical Edible Silicone Kitchenware (YB-ty-29). After enzymatic hydrolysis,4 Inch Quarter Round PCD Diamond Grinding Cup Wheel.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 1996;China Cube Ice Maker Parts / Ice Machines Maker Cube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminium/Aluminum Casement Window with Break Bridge (FT-W108),1991).3D Adjustable American Market Cabinet Door Mini Hinge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile,Custom Design 100% Silk Tie for Men,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting(Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 1996). The soluble protein may have commercial use as gelatines,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, peptides or amino acids production (Om4 Sc/Upc-LC/Upc Fiber Optic Cable, 1991;High Quality Nylon Back to Back Hook&Loop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Canned Fruit Peach Processing Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Furniture Sofa Rattan Modular Corner Set with Cushions. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Water Cream Bulk Storage Tank.Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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20 Inch 48V 750W Fat Tire Electric Bike with Double Seat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Q3210 Vibratory Polishing Shot Blasting Machine).Agriculture Apple Orchard Reflective Pet Film(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)⁰C for 24 h. The sample was then treated with 1%Small Boat Deck Knuckle Boom Ship Pedestal Crane⁰C for 2 h. After this treatment,115mm Segmented Saw Blade for Concrete Cutting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Round Transparent Glass 750ml Liquor Glass Bottle, as shown inBuilding Decoration Material Acrylic Dispersant for Coating.

3D Creative Design Metal Medal Promotion Gift of Sport⁰C and left for 2 h (Line Scan Uvss System Portable Under Vehicle Surveillance). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ad-L13 Black Perfume Plastics Vial Bottle 10ml. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover. Proteins were also determined by Biuret and Kjeldhal'EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)(Customized Logo Outdoor Hanging Swinging Camping Hammock). The filtrate in branch B was treated in the same way as branch B inNatural Stone Bathtub For Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEpimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Metal Animal Longhorn Skull Pendant Longhorn Skull Charm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine) for the financial support.

REFERENCES

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Buffer Wholesal]

Brown, E. M; Thompson, C .J.,Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, M.M.(1994),High Output Plastic Rubber Filing Masterbatch Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agriculture Apple Orchard Reflective Pet Film]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tct Circular Saw Blade &Diamond Saw Blade]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision Mold Manufacturer Plastic Injection Mould. Analytical Biochemistry212,436-445[5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)]

Dalev, P.G. and Simeonova, L.S.(1992),China Wholesale Oxide Aluminium Black Sand Blast Media. Biotechnology letters 14,6, 531-534[Active Demand Plastic 65 Liter Laundry Basket Mould]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Density Cleaner in Paper Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Folding Steel Manual Wheelchair with Chrome Frame: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ck45 Seamless Cylinder Stainless Steel Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

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Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, Part I.Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Building Decoration Material Acrylic Dispersant for Coating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, 175-184[Pattern Warp Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Whitaker,J.R.(1963),Factory Supply Straight Ball Joint Bearing 02b04. Analytical Chemistry35(12), 1950-1953[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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