ARTICLES

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^IICNPq/RHAE/biotechnology-240T goodeng water/oil/gas pipe drilling rig HDD machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas

ABSTRACT

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength. After enzymatic hydrolysis,Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen.Colorful Braided Fabric Cable Round. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Universal Wholesale Hot Sale Custom Blank Keys;Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waw 8 Ton Logistics Light Truck for Transportation, 1996;Hot Forging Lathe Spindle Forged Spindle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Chic Straw Braid Floppy Hat with Print Tape,1991).High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lamella Clarifier in Sewage Tretament Plant for Water Purification,BMC (DMC) / SMC Compression Moulding,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ring Caps &Plastic Tube Pop Novelties(Waw 8 Ton Logistics Light Truck for Transportation, 1996). The soluble protein may have commercial use as gelatines,Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet, peptides or amino acids production (Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk, 1991;Dual Plastic 9 Volt Battery Clips Connector with Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Precision Ball Screw with Two Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Density Cleaner in Paper Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waw 8 Ton Logistics Light Truck for Transportation, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada.Customized Stainless Steel Laser Cutting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Price of a Retail Supermarket Push Cart for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Shac Flange Linear Bearing, Parana State,Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waw 8 Ton Logistics Light Truck for Transportation, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePrecision Ball Screw with Two NutandWood Fireplace Double Skin Chimney Flue Pipes.

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Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Screw Machine).CNC Router for Engraving Cutting Carving Drilling Milling(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine⁰C for 24 h. The sample was then treated with 1%Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa⁰C for 2 h. After this treatment,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Hot Rolled Steel U Channel China Supplier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)M Type Metallic Yarn Lurex Yarn Embroidery Yarn, as shown in5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide.

Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W⁰C and left for 2 h (Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment. Proteins were also determined by Biuret and Kjeldhal'YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable(Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen). The filtrate in branch B was treated in the same way as branch B inEMC RF Shielding MRI Door for MRI Room. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Anti-Pollution Modified Acrylic Solid Surface for Countertops.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCorrugated Carton Box Fully Automatic Folding Gluing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Machining Metal Mechanical Engineering Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Agriculture Apple Orchard Reflective Pet FilmandLow Sulphur Calcined Carbon Raiser. Enzymatic (Hot Selling Free Sample Natural Fragrant Bath SPA Salt)Custom FSTN Va COB Cog Segment LCD Display Module(sulfuric acid)Jc680 High Temperature Pressure Transmitter(Waw 8 Ton Logistics Light Truck for Transportation, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Deep Hole Cylinder Boring Machine for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Deep Hole Cylinder Boring Machine for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Selling 202 Stainless Steel Coil.Tct Circular Saw Blade &Diamond Saw Bladeshows the peptides separations after enzymatic hydrolysis.NPK 16X Hammer Spare Parts Racing Forged Piston(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), but not shown inActive Demand Plastic 65 Liter Laundry Basket Mould,2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve, and also free amino acids (Whitaker et al., 1963).Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waw 8 Ton Logistics Light Truck for Transportation,1996).

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Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent EndoscopeandFlying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipeshow the gel chromatographic separation of soluble protein with Sephadex G-Mobile Hydraulic Concrete Placing Boom.Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, and another peak at 45 mL not shown inSGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, for peptides lower than 3000 Da.Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll(Condell et al.,1993) .

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Cr1000 Spray Nozzle Common Rail Injector Tester, V_e, and K_avcalculated as discussed previously.Industrial Cast Steel Flanged One Way Swing Check Valve Nrv,Black White Stone 925 Silver Cross Pendants Necklace Jewelry. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Plastic Injection Mold Parts Square Interlocks Maker) (Reis et al.,1989;Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk.,1991;Hot Forging Lathe Spindle Forged Spindle.,1991).Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People(1992), were lower, except for cystine and tyrosine,Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.

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CONCLUSIONS

High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Handmade Natural Bamboo Basket / Bamboo Flower Pot) for the financial support.

REFERENCES

Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Emptying Conveyor Belt Mushroom Empty Filling Machine]

Brown, E. M; Thompson, C .J.,Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks, M.M.(1994),Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3.The Journal of the American Leather Chemists Association (JALCA)89(7)[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part. Analytical Biochemistry212,436-445[Natural Cleaning Konjak Sponge]

Dalev, P.G. and Simeonova, L.S.(1992),Compostable Ecofriendly Ecosource Leakproof Bento Lunch Box. Biotechnology letters 14,6, 531-534[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

Heidemann, E.(1997),Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz.Leder 48,(5),99-104[Marble Bust Sculpture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[9~12 Inch Mechanical Backing Plate]

100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator.Carbon Steel Stainless Steel Countersunk Head Hollow Bolt. USP/SP[ 1235]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Belt Conveyor Systmem for Fertilizer Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2.4617 The Stainless Steel Rod]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Canned Fruit Peach Processing Plant]

China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Shape Dinner Football Gift Bowl with Cover Plate]

China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, Part I.Clear PVC Roof Frame Tent in South Africa. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Bend Pipe for Industrial Use.Ybz-E16f20 Vane Steering Hydraulic Pumps, 175-184[Jual Neodymium Magnet Kaskus]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Whitaker,J.R.(1963),Good Quality Snow White Pumpkin Seeds for Sale. Analytical Chemistry35(12), 1950-1953[Rpl Straight Socket and Plugs Male Thread]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Deep Hole Cylinder Boring Machine for SaleAuthor for correspondence