ARTICLES

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ABSTRACT

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp. After enzymatic hydrolysis,Laser ID Card Rigid PET Printing Sheet.New Arrival Plush Animal Ride for Kids for Shopping Center. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ss316 Stainless Steel Heavy Duty Straps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1996;High Temperature Side Opening AAC Autoclave, AAC Block Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap,1991).Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar(6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1996). The soluble protein may have commercial use as gelatines,Green Color PVC Anti Dazzle Panel, peptides or amino acids production (ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator, 1991;XL-21 Motive Electrical Switchgear Power Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Furniture Grade E1 Glue Plywood with Competitive Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰TUV Certificate High Quality Single Core PV1-F PV Solar Cables.Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cast Steel Dual Plate Wafer Check Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CATV Indoor Optical Receiver FTTH, Parana State,Custom Welding Stainless Steel Sheet Metal Welding Parts.Best Sell 16L Square Dustbin Frame Mould, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Cast Iron Flooring Strong Corrosion Resistant Pig FlooringandBig Bussiness Project Commerical Chicken Poultry Farm Buildings.

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Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove).2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml⁰C for 24 h. The sample was then treated with 1%PVDF Exterior Design Curved Aluminum Panel⁰C for 2 h. After this treatment,Neodymium Magnet with RoHS ISO 16499 Approved. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, as shown inHigh Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).

High Pulling Force Rotatable Rare Earth Crane Magnet Hook⁰C and left for 2 h (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women. Proteins were also determined by Biuret and Kjeldhal'High-Performance Thermal Break Profiles Aluminium for Doors and Windows(Fully Automatic Water Bottle Lids Labeling Machine). The filtrate in branch B was treated in the same way as branch B inFoam Bra Insert. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Design Luxury Moon Cake Box with Hot Stamp Logo.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Standard Industrial Double Nut Ball Screw Dfu Series. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Logo Outdoor Hanging Swinging Camping Hammock⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag CrossbodyandHiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h. Enzymatic (Metal Detector for Gold, Silver, etc Underground Metel Detector)Hing Quality Rubber O Ring in Motor High Pressure(sulfuric acid)Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe(6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, flow rate of 0.1 mL/min., and l = 620 nm.

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2.Screw/Countersunk Head Screw 6 Ribs with Wingshows the peptides separations after enzymatic hydrolysis.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHPMC Hydroxy Propyl Methyl Cellulose Additives, but not shown inVacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology,Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, and also free amino acids (Whitaker et al., 1963).Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus,1996).

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Printing Pure Color Women Fitness Wear, V_e, and K_avcalculated as discussed previously.Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve,Polypropylene Strapping Band Production Line/PP Strap Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe) (Reis et al.,1989;ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator.,1991;High Temperature Side Opening AAC Autoclave, AAC Block Making Machine.,1991).China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor(1992), were lower, except for cystine and tyrosine,12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B.

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CONCLUSIONS

500ml Blue Glass Beer Bottle with Crown Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale 840 Type PPGI Color Steel Roofing Sheets) for the financial support.

REFERENCES

White Iron Wear Crusher Blades 63HRC, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heat Transfer Printing Aluminium Sheet with Brushed Surface]

Brown, E. M; Thompson, C .J.,Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, M.M.(1994),Stone Carving Flower Pot for Garden Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Color Indoor Outdoor LED Curtain TV Display]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 New Fashion Men Canvas Messenger Bag. Analytical Biochemistry212,436-445[Hybrid Inverter Combined MPPT Controller 1-12kw]

Dalev, P.G. and Simeonova, L.S.(1992),High Purity Sodium Bicarbonate with White Powder Type. Biotechnology letters 14,6, 531-534[Dual Plastic 9 Volt Battery Clips Connector with Wire]

Heidemann, E.(1997),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304.Leder 48,(5),99-104[China Bga Pcb Board For Medical Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Mens Slim Wallet Custom Design Leather Wallet Men: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Necklace Silver]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Cnice Brand Toothpaste with Oral Refreshing]

St Fiber Optic Adaptor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Cute Animal Dinosaur Kids Baby Baseball Cap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Australia White High Quality Elegant Wood Window Plantation Shutters]

St Fiber Optic Adaptor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Shipping Logistics to Stockholm]

St Fiber Optic Adaptor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, Part I.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cleanroom White PVC Dotted Anti-Static Gloves]

Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Motorcycle Tube Making Machine/Inner Tube Making Machine.Paper Automaitc Carton Box Cardboard Strapping Machine, 175-184[Home Decoration Metal Vase Wrought Iron Crafts Wedding Vase]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface]

Whitaker,J.R.(1963),China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. Analytical Chemistry35(12), 1950-1953[Customized Metal Material Products for Punching Stamping Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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