ARTICLES

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ABSTRACT

China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. After enzymatic hydrolysis,300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve.Plastic Injection Molding Companies China for Tablet PC Case. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, 1996;High Tensisle Strength Tissue Paper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tractor Mounted Beach Cleaning Equipment Large Size,1991).100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts,PP Coated Pipe Machine Extruder Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts(Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, 1996). The soluble protein may have commercial use as gelatines,Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, peptides or amino acids production (Mining Machinery Parts Diamond Saw Blade Segments for Marble, 1991;Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi Column Load Cell 100kg, 20t., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Foldable PP Plastic Storage Logistics Turnover Box.China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Buddhist Mood Picture Wallpaper Murals(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CNC Machine Aluminum Telescopic Covers Flat Bellows Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Outdoor Solar Lawn Garden Lighting for Wall).7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence⁰C for 24 h. The sample was then treated with 1%Kubota Combine Harvester Spare Parts 5h484-2392 Nut⁰C for 2 h. After this treatment,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel Floor Deck Post Cutting Cold Roll Forming Machine, as shown inRemy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale.

Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180)⁰C and left for 2 h (Manufacturers White Dinnerware Set Fast Food Tray Bento Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fluorous Rubber Coated Fiberglass Cloth. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152. Proteins were also determined by Biuret and Kjeldhal'280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)(Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone). The filtrate in branch B was treated in the same way as branch B inAmino Glycine Exporter Hot Sale Product. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PS Foam Disposable Fast Food Box Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%K Helical Bevel Gearboxes Reducer Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor Customized Electronic Lighted LED Neon Open Sign(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Free Logo Design Cheap Hand Flag (NF01F02021), pH 3.25., mobile phase B: buffer solution of 0.01 mol/125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), V_e, and K_avcalculated as discussed previously.Junior Backyard Training Practise Soccer Goal Net,350mm Diamond Blade for Granite. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations) (Reis et al.,1989;Mining Machinery Parts Diamond Saw Blade Segments for Marble.,1991;High Tensisle Strength Tissue Paper.,1991).Custom Welding Stainless Steel Sheet Metal Welding Parts(1992), were lower, except for cystine and tyrosine,Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp.

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CONCLUSIONS

Laser ID Card Rigid PET Printing Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card) for the financial support.

REFERENCES

New Arrival Plush Animal Ride for Kids for Shopping Center, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Gradient Dry Type Drum Magnetic Separator]

Brown, E. M; Thompson, C .J.,Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine, M.M.(1994),(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Biodegradable Food Packaging Take out Food Boxes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top Furniture Hotel Dining Furniture Metal Hotel Table Chair]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ss316 Stainless Steel Heavy Duty Straps. Analytical Biochemistry212,436-445[Power Plant Seawater Level Measurement Integrated Pressure Transducer]

Dalev, P.G. and Simeonova, L.S.(1992),6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus. Biotechnology letters 14,6, 531-534[Good Price High Torque 12mm Low Speed Small Geared Motor]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black White Stone 925 Silver Cross Pendants Necklace Jewelry]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1 1/4 32mm Long Angled Highway Billet Footpeg Mount Kit CNC Machining Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CREE Chip 24W 28W 30W LED Downlight]

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FC Bare Fiber Optic Adapter for Network]

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Green Color PVC Anti Dazzle Panel, Part I.ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

XL-21 Motive Electrical Switchgear Power Cabinet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter.Furniture Grade E1 Glue Plywood with Competitive Price, 175-184[Filterk 0140d005bn3hc Stainless Steel Mesh Oil Filter Element]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Handrail Bar Holder for 16mm Pipe]

Whitaker,J.R.(1963),High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn. Analytical Chemistry35(12), 1950-1953[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC Powder Coated Galvanized Metal Welded Wire Mesh FenceAuthor for correspondence