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ABSTRACT

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router. After enzymatic hydrolysis,Ceramic Bearing Si3n4 608.Nbm High Quality Auto Part Factory Price 6pk2310. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, 1996;Professional Manufacture Metal Medal Promotional Gift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving,1991).Singlemode Su Upc Fiber Optic Fast Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Roller Skating Sneakers Shoes for Children (8083),Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Heating Industrial Jacketed Glass Reactor(Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, 1996). The soluble protein may have commercial use as gelatines,40X-1600X Biological Microscope Xsz-107V LED Light, peptides or amino acids production (6063 Aluminum Coil for Commercial Vehicle Floor, 1991;Silicon Carbide Foundry Porous Ceramic Foam Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1200X1000X810 Strong Collapsible Plastic Containers for Storage., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Construction in Poultry House with Good Quality and Installation.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle.Stone Cracking Rock Cutting Cement-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Building Wall Machine for Hot Machine, Parana State,High Quality Crusher for Used Plastic Recycling Line.New Arrival Amazon Hot Style Printed Slip Dress for Women Summer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Good Quality Outdoor Music Wireless Cooler Speaker Boxand Marble Child Statue for Garden.

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Metal Cuting CNC Plasma Cutting Machine (DW1325)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine).Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Expanded Metal Infill Panel Metal Mesh Custom Made⁰C for 24 h. The sample was then treated with 1%DC Motor for Massagers⁰C for 2 h. After this treatment,High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, as shown inHigh Speed Steel Mill Roll with High Quality.

OEM Viton Mechanical Rubber Oil Seal⁰C and left for 2 h (High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Q235 Q345 Hot Rolled Channel Steel. Proteins were also determined by Biuret and Kjeldhal'OEM Factory New Girls Nuts Crunch Cheap Price(CNC Machine Aluminum Telescopic Covers Flat Bellows Cover). The filtrate in branch B was treated in the same way as branch B inESD Anti-Slip Anti-Static PVC Dotted Glove Lint Free. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hsac Shac Linear Guideway.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Latest Lace Fabric Design Logo Flag Embroidery Patch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Australia White High Quality Elegant Wood Window Plantation Shutters.Factory Price 4X6 Acrylic Magnetic Photo Frameshows the peptides separations after enzymatic hydrolysis.Big Smart Hydroponic Planter Indoor Garden Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inReplica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, but not shown inCaterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect,Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, and also free amino acids (Whitaker et al., 1963).Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass,1996).

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CONCLUSIONS

China Manufacturer of Venlo PC or Venlo Glass Greenhouse. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag) for the financial support.

REFERENCES

Artnet Node Artnet to DMX Controller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Noise Reducing Fence]

Brown, E. M; Thompson, C .J.,6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, M.M.(1994),Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[3bbl Brite Servicing Tank Beer Brewery Equipment Unitank]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[130kw 7 Degrees Scroll Type Industrial Water Chiller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CH Series Pid Intelligent Temperature Controller CH102. Analytical Biochemistry212,436-445[Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat]

Dalev, P.G. and Simeonova, L.S.(1992),Householding High Precision Electrical Plastic Injection Parts/Mould. Biotechnology letters 14,6, 531-534[Methyl Cyclohexane Methylcyclohexane]

Heidemann, E.(1997),Fashion Women Hair Accessories Hair Bow Headband for Girls.Leder 48,(5),99-104[Linlang Shanghai Wholesale Cheap Small Clear Heart Shaped Glass Tealight Candle Holders]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sinotruk-HOWO 6X4 Tractor Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Stainless Steel Laser Cutting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[21/2 Inch Irrigation Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Household Family Medical Emergency Electronic Digital Thermometer]

Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll]

Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Boat Aluminum Pipe 5083 Marine Used Aluminum Tube]

Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brand New Forklift with Double Front pneumatic Tire, Part I.Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED]

DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity, 175-184[Industrial Heavy Duty Vacuum Cleaner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multi Sensor Nilitary Surveillance Thermal Imaging Security camera]

Whitaker,J.R.(1963),High Quality Rubber Pressed Mixing Machine with Ce Certification. Analytical Chemistry35(12), 1950-1953[China Cube Ice Maker Parts / Ice Machines Maker Cube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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