ARTICLES

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ABSTRACT

Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead. After enzymatic hydrolysis,Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass.Professional Manufacture Metal Medal Promotional Gift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving;Singlemode Su Upc Fiber Optic Fast Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Roller Skating Sneakers Shoes for Children (8083), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, 1996;Electric Heating Industrial Jacketed Glass Reactor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (40X-1600X Biological Microscope Xsz-107V LED Light,1991).6063 Aluminum Coil for Commercial Vehicle Floor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silicon Carbide Foundry Porous Ceramic Foam Filter,1200X1000X810 Strong Collapsible Plastic Containers for Storage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Construction in Poultry House with Good Quality and Installation(Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, 1996). The soluble protein may have commercial use as gelatines,Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, peptides or amino acids production (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle, 1991;Stone Cracking Rock Cutting Cement, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag.Aluminum Hot Forging Accessories Machining Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Building Wall Machine for Hot Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DC Motor for Massagers, Parana State,High Quality Mini LED Flood Light SMD Flood Light 50W.High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Women Hair Accessories Hair Bow Headband for GirlsandE-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.

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Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Cnice Brand Toothpaste with Oral Refreshing).Custom Good Quality Outdoor Music Wireless Cooler Speaker Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Viton Mechanical Rubber Oil Seal⁰C for 24 h. The sample was then treated with 1%Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric⁰C for 2 h. After this treatment,Q235 Q345 Hot Rolled Channel Steel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Factory New Girls Nuts Crunch Cheap Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hsac Shac Linear Guideway, as shown inAustralia White High Quality Elegant Wood Window Plantation Shutters.

Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses⁰C and left for 2 h (China Shipping Logistics to Stockholm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pure Water Cutting Head Assy for Waterjet Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat(Cleanroom White PVC Dotted Anti-Static Gloves). The filtrate in branch B was treated in the same way as branch B inToddler Visor Hat Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Latest Lace Fabric Design Logo Flag Embroidery Patch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New H Type Battery Cage System for Layer Chicken Farm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Calcined Petroleum Coke as Best Recarburizer in Iron Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/X15 Power Monitor Two Way Live Stage Monitor Speaker System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Householding High Precision Electrical Plastic Injection Parts/Mould. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant) for the financial support.

REFERENCES

Fashion Women Hair Accessories Hair Bow Headband for Girls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Oled Panel Display]

Brown, E. M; Thompson, C .J.,Round Hole Carbon Steel Pipe Perforated for Filter, M.M.(1994),Professional Manufacturer Stainless Steel Floor Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kt Board Acrylic CCD Camera CNC Knife Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels. Analytical Biochemistry212,436-445[Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. Biotechnology letters 14,6, 531-534[3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer]

Heidemann, E.(1997),Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips.Leder 48,(5),99-104[Automatic Gas Beverage Canning Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waw 8 Ton Logistics Light Truck for Transportation]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[GLHK-3 Pipe Welding Roller Pipe Welding Rotator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Artnet Node Artnet to DMX Controller]

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling Custom Sheet Metal Fabrication]

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Boat Deck Knuckle Boom Ship Pedestal Crane]

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Rubber Pressed Mixing Machine with Ce Certification, Part I.Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Sides Clear White Frosted Acrylic Sheet]

4K Giant Whiteboard Interactive Panel for University Whiteboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid.Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), 175-184[Brass Cufflink]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part]

Whitaker,J.R.(1963),Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting. Analytical Chemistry35(12), 1950-1953[Wrinkle Effect Crease UV Drying Machine UV Curing System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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