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ABSTRACT

High Quality Rubber Pressed Mixing Machine with Ce Certification. After enzymatic hydrolysis,Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600.4K Giant Whiteboard Interactive Panel for University Whiteboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid;Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, 1996;Practical Edible Silicone Kitchenware (YB-ty-29)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4 Inch Quarter Round PCD Diamond Grinding Cup Wheel,1991).High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG),Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)(Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, 1996). The soluble protein may have commercial use as gelatines,Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, peptides or amino acids production (China Cube Ice Maker Parts / Ice Machines Maker Cube, 1991;Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Adjustable American Market Cabinet Door Mini Hinge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Design 100% Silk Tie for Men. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting.Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Om4 Sc/Upc-LC/Upc Fiber Optic Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Three Phase 35kv Current Transformer for Indoor).Seasoned Cast Iron South Africa Three Legged Potjie Pot(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1.0g Water and Air Ozone Generator with Silicone Tube⁰C for 24 h. The sample was then treated with 1%Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine⁰C for 2 h. After this treatment,16A/24A High Speed Wire Cable Braiding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), as shown inStainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Round Transparent Glass 750ml Liquor Glass Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ad-L13 Black Perfume Plastics Vial Bottle 10ml⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate) for the financial support.

REFERENCES

Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Patio Furniture Dining Set]

Brown, E. M; Thompson, C .J.,Outdoor Fitness Equipment Swing (JMH-03), M.M.(1994),Sheet Metal Distribution Cabinet with High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Real Leather Wallet Mens Designer Leather Wallets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. Analytical Biochemistry212,436-445[ 60 Hz]

Dalev, P.G. and Simeonova, L.S.(1992),New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. Biotechnology letters 14,6, 531-534[Stone Cracking Rock Cutting Cement]

Heidemann, E.(1997),Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting.Leder 48,(5),99-104[Co2 Copper Coated Welding Material]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Corn Feed Bag Rice Bag 10]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Vehicle Charging-Station]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

High Output Plastic Rubber Filing Masterbatch Extruder Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Precision Mold Manufacturer Plastic Injection Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pepper Peanut Sauce Colliod Miller Grinding Machine]

High Output Plastic Rubber Filing Masterbatch Extruder Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor]

High Output Plastic Rubber Filing Masterbatch Extruder Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Wholesale Oxide Aluminium Black Sand Blast Media, Part I.Welded Stud Anchor Chain for Ship. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1]

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Line Scan Uvss System Portable Under Vehicle Surveillance.Folding Steel Manual Wheelchair with Chrome Frame, 175-184[3.1*40 Concrete Nails]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump]

Whitaker,J.R.(1963),Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. Analytical Chemistry35(12), 1950-1953[Painting Road Marking Vehicles Machine for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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