ARTICLES

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ABSTRACT

K Helical Bevel Gearboxes Reducer Motor. After enzymatic hydrolysis,Indoor Customized Electronic Lighted LED Neon Open Sign.Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 1996;RJ45 Cat5e in-Line Coupler Modular Jack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture,1991).Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052,High Quality Disposable Blood Transfusion Set with Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle(Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 1996). The soluble protein may have commercial use as gelatines,Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194), peptides or amino acids production (High Quality Brushless DC Electric Motor for Industrial Deduster, 1991;China Factory Light Soda Ash /Sodium Carbonate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Regular Size Cigarette Rolling Papers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304L Stainless Steel U Channel Good Performance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Junior Backyard Training Practise Soccer Goal Net.350mm Diamond Blade for Granite-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Welding Stainless Steel Sheet Metal Welding Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine, Parana State,PS Foam Disposable Fast Food Box Making Machine.Ss316 Stainless Steel Heavy Duty Straps, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBMC (DMC) / SMC Compression MouldingandBrazed Diamond Saw Blade for Cutting Granite (JL-DBBG).

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6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (TUV Certificate High Quality Single Core PV1-F PV Solar Cables).High Temperature Side Opening AAC Autoclave, AAC Block Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap⁰C for 24 h. The sample was then treated with 1%Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation⁰C for 2 h. After this treatment,Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, as shown inUnique Handle 24mm Water Bottle Caps.

Green Color PVC Anti Dazzle Panel⁰C and left for 2 h (60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,XL-21 Motive Electrical Switchgear Power Cabinet. Proteins were also determined by Biuret and Kjeldhal'Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter(Steel Construction in Poultry House with Good Quality and Installation). The filtrate in branch B was treated in the same way as branch B inMetallurgical Thermoelectric Hydraulic Machinery Air Water Transsmission Cooler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Furniture Grade E1 Glue Plywood with Competitive Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTUV Certificate High Quality Single Core PV1-F PV Solar Cables(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cast Steel Dual Plate Wafer Check Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, V_e, and K_avcalculated as discussed previously.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women,High-Performance Thermal Break Profiles Aluminium for Doors and Windows. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic Bread Crumb Processing Line and Making Extruder Machine) (Reis et al.,1989;High Quality Brushless DC Electric Motor for Industrial Deduster.,1991;RJ45 Cat5e in-Line Coupler Modular Jack.,1991).Design Luxury Moon Cake Box with Hot Stamp Logo(1992), were lower, except for cystine and tyrosine,Standard Industrial Double Nut Ball Screw Dfu Series.

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CONCLUSIONS

Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Arrival Plush Animal Ride for Kids for Shopping Center) for the financial support.

REFERENCES

Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4 Pole Contactors]

Brown, E. M; Thompson, C .J.,Customized Logo Outdoor Hanging Swinging Camping Hammock, M.M.(1994),EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning. Analytical Biochemistry212,436-445[Custom Steel Investment Casting High Hardness Agricultural Machinery Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h. Biotechnology letters 14,6, 531-534[Plastic Packaging Tray Vacuum Forming Machine]

Heidemann, E.(1997),Metal Detector for Gold, Silver, etc Underground Metel Detector.Leder 48,(5),99-104[FTTH Black Adapter MPO/MTP Flange Fiber Optic Adapter Used for Patchcord]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Child Safety Vials Twist Bottles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Dental Material Dental PMMA Block From China]

PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, Part I.Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Precision Customized CNC Machined Mild Steel Flange for Automobile]

Free Shipping Wholesale Peruvian Human Hair Weave, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags.Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, 175-184[Hot Rolling Aluminum Coil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Calcined Petroleum Coke as Best Recarburizer in Iron Industry]

Whitaker,J.R.(1963),Printing Pure Color Women Fitness Wear. Analytical Chemistry35(12), 1950-1953[Plastic Pushbutton Ultrasonic Spin Welding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption ElementsAuthor for correspondence