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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale

ABSTRACT

800mm Length 600n Load Tension Gas Spring with Metal Eyelet. After enzymatic hydrolysis,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant;SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100 Liter Cooking Pots for Jam, 1996;Food Grade Customized Metal Candy Can Square Mint Tin Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (600X600 mm Ugr 19 LED Panel Lights,1991).Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine,Round Plastic Hanging Basket (KD7014),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag(100 Liter Cooking Pots for Jam, 1996). The soluble protein may have commercial use as gelatines,China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, peptides or amino acids production (10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 1991;New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Setractable Rail Protection Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Natural Birch F&B Veneer Furniture Plywood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100 Liter Cooking Pots for Jam, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Tire Repair Tool Kit, Tire Repair Strip.High Performance 915MHz PCB Antenna-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:GRP/FRP Profile Structures/Fiberglass Pultrusion Product(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System, Parana State,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100 Liter Cooking Pots for Jam, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Window System Thermal Break Strip (HK Stype35.3mm)andAuto Parts Cross Propeller Shaft for Mercedes Benz 12062X.

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Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ampoule Sealer Machine Hydrogen Oxygen Gas Generator).Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Theme Park Inflatable Bouncer for Sale (BJ-B16)⁰C for 24 h. The sample was then treated with 1%Muliti-Size Light Hot Stamping Foil for PS Frame⁰C for 2 h. After this treatment,Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China 1 & 100 RMB Acrylic Currence Frame, as shown in55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk.

1 Ton Toyo Type Manual Chain Block⁰C and left for 2 h (IS Type Cast Iron Chemical Industrial Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Spark Resistant Construction Axial Fan with Fixed Pitch Blade. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl. Proteins were also determined by Biuret and Kjeldhal'3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(Starter Motor 0001367034 12V 3KW 9T for Mercedes). The filtrate in branch B was treated in the same way as branch B inAir Cooled Piston Air Compressor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Precision CNC Milling DIN69871. Mas403 for CNC Machinist. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCaterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport CourtandPrecision Customized CNC Machined Mild Steel Flange for Automobile. Enzymatic (Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing)Metal Container Packaging for Edible Oil Tin Cans Storage Boxes(sulfuric acid)Melee Custom Plastic Mould New Design(100 Liter Cooking Pots for Jam, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light) for the financial support.

REFERENCES

Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kerala Stone Coated Metal Roof Tile]

Brown, E. M; Thompson, C .J.,New Electric Piston Pump Coating Machine PT6900, M.M.(1994),200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Neodymium Magnet Pot Assembly with Strong Weight]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Clear PVC Roof Frame Tent in South Africa]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. Analytical Biochemistry212,436-445[China Soundproof Construction Foam PVC/WPC Board]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Crusher for Used Plastic Recycling Line. Biotechnology letters 14,6, 531-534[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Heidemann, E.(1997),300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.Leder 48,(5),99-104[www.febwebbing.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Wire Harness for Various Application]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Quality Tube Welding Stainless Steel Pipe Mill]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

High Quality Mini LED Flood Light SMD Flood Light 50W, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

High Quality Mini LED Flood Light SMD Flood Light 50W,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

High Quality Mini LED Flood Light SMD Flood Light 50W, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, Part I.Professional Manufacture Metal Medal Promotional Gift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Grade Customized Metal Candy Can Square Mint Tin Box]

Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Singlemode Su Upc Fiber Optic Fast Connector.Roller Skating Sneakers Shoes for Children (8083), 175-184[Child Car Seat Booster]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Whitaker,J.R.(1963),Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. Analytical Chemistry35(12), 1950-1953[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ybz-E16f20 Vane Steering Hydraulic PumpsAuthor for correspondence